IP Library Granted Patent US 10,478,439
Granted Patent B2
US 10,478,439 · App. 15/965,114 · Granted Nov 19, 2019

Use of inhibitors of bruton's tyrosine kinase (Btk)

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Quick Facts
Patent No.
US 10,478,439
App. No.
15/965,114
Granted
Nov 19, 2019
Kind
B2
Abstract

Disclosed herein are methods for treating a cancer comprising: a. administering a Btk inhibitor to a subject sufficient to result in an increase or appearance in the blood of a subpopulation of lymphocytes defined by immunophenotyping; b. determining the expression profile of one or more biomarkers from one or more subpopulation of lymphocytes; and c. administering a second agent based on the determined expression profile.

Claims (22)

1. A method of inhibiting proliferation and survival of activated B-cells in a human subject suffering from a B-cell proliferative disorder, comprising: orally administering to the human subject suffering from a B-cell proliferative disorder a therapeutically effective amount of 1-((R)-3-(4-amino-3-(4-phenoxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl)piperidin-1-yl)prop-2-en-1-one (Compound 13), on a continuous once-daily regimen until progression of the disorder or unacceptable toxicity,

wherein said administration of the therapeutically effective amount results in an AUC (0-24) of >about 100 ng*h/ml; and

wherein said administration of the therapeutically effective amount results in >90% of the Btk active sites in the peripheral blood mononuclear cells of the human subject being occupied by Compound 13 twenty-four hours following said administration.

2. The method of claim 1 , wherein the once daily regimen is continued for at least 6 months.

3. The method of claim 1 , wherein the B-cell proliferative disorder is a hematological malignancy.

4. The method of claim 3 , wherein the hematological malignancy is a non-Hodgkin lymphoma.

5. The method of claim 3 , wherein the hematological malignancy is selected from the group consisting of chronic lymphocytic leukemia, small lymphocytic lymphoma, and Waldenstrom's macroglobulinemia.

6. The method of claim 3 , wherein the hematological malignancy is selected from the group consisting of mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, and ABC-diffuse large B-cell lymphoma.

7. The method of claim 1 , wherein the human subject has previously undergone a bone marrow transplant.

8. The method of claim 7 , wherein the human subject has previously undergone an autologous bone marrow transplant.

9. The method of claim 3 , wherein the hematological malignancy is relapsed or refractory.

10. A method of inhibiting proliferation and survival of activated B-cells in a human subject suffering from a B-cell proliferative disorder, comprising:

orally administering to the human subject suffering from a B-cell proliferative disorder a therapeutically effective amount of 1-((R)-3-(4-amino-3-(4-phenoxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl)piperidin-1-yl)prop-2-en-1-one (Compound 13),

wherein the therapeutically effective amount of Compound 13 is an amount that results in >90% of the Btk active sites in the peripheral blood mononuclear cells of the human subject being occupied by Compound 13 twenty-four hours following said administration, and

wherein proliferation and survival of the activated B-cells in the human are inhibited.

11. The method of claim 10 , wherein the B-cell proliferative disorder is a hematological malignancy.

12. The method of claim 11 , wherein the hematological malignancy is a non-Hodgkin lymphoma.

13. The method of claim 11 , wherein the hematological malignancy is selected from the group consisting of chronic lymphocytic leukemia, small lymphocytic lymphoma, and Waldenstrom's macroglobulinemia.

14. The method of claim 11 , wherein the hematological malignancy is selected from the group consisting of mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, and ABC-diffuse large B-cell lymphoma.

15. The method of claim 10 , wherein the human subject has previously undergone a bone marrow transplant.

16. The method of claim 15 , wherein the human subject has previously undergone an autologous bone marrow transplant.

17. The method of claim 11 , wherein the hematological malignancy is relapsed or refractory.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 2, 2019
From: HONIGBERG, LEE; LOURY, DAVID J.
To: PHARMACYCLICS LLC
Reel/Frame 049943/0670 →