Detecting gastric neoplasm
Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting premalignant and malignant neoplasms such as gastric cancer.
1. A method comprising:
a) extracting genomic DNA from a biological sample of a human individual suspected of having or having a neoplasm of the gastrointestinal system;
b) treating the extracted genomic DNA with bisulfite;
c) amplifying the bisulfite-treated genomic DNA using a pair of primers specific for each of ARHGEF4 and ELMO1; and
d) measuring the methylation level of the CpG site for each of ARHGEF4 and ELMO1 by methylation-specific PCR, quantitative methylation specific PCR, methylation-sensitive DNA restriction enzyme analysis, or bisulfite genomic sequencing PCR.
2. The method of claim 1 , wherein the biological sample is a tissue sample, a plasma sample, or a blood sample.
3. The method of claim 1 ,
wherein ARHGEF4 is a genetic region having chromosome 2 coordinates 131797843-131797938, and
wherein ELMO1 is a genetic region having chromosome 7 coordinates 37487867-37488498.