Operatorless particle processing systems and methods
The present disclosure provides improved particle processing (e.g., cytometry and/or cell purification) systems and methods that can operate in an autonomous fashion. More particularly, the present disclosure provides for assemblies, systems and methods for analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting and/or enriching) particles (e.g., cells, microscopic particles, etc.) where human intervention is not required and/or is minimized. The systems, assemblies and methods of the present disclosure advantageously improve run performance of particle processing systems (e.g., cell purification systems, cytometers) by significantly reducing and/or substantially eliminating the burden of operation for human intervention by automating numerous functions, features and/or steps of the disclosed systems and methods.
1. A method for automatically aligning a microfluidic chip for a particle processing system, the method comprising:
receiving a microfluidic chip into a chip receptacle;
illuminating the microfluidic chip with a radiation source;
detecting extinction signals;
coarsely scanning the chip in a first-direction, a second-direction, or both the first and second-direction to generate extinction signals;
determining a coarse slant angle based on a dimension determined from the coarse scanning and a known dimension of the microfluidic chip;
rotating the microfluidic chip by the negative of the coarse slant angle;
finely scanning the chip in a first-direction, a second-direction, or both the first- and second-direction to generate extinction signals;
determining a fine slant angle based on a dimension determined from the fine scanning and a known dimension of the microfluidic chip; and
rotating the microfluidic chip by the negative of the fine slant angle.
2. The method of claim 1 , wherein the microfluidic chip is removably received into the chip receptacle.
3. The method of claim 1 , wherein the microfluidic chip includes a pair of separated fiducials and the step of coarsely scanning includes scanning at least one of the fiducials.
4. The method of claim 1 , wherein a coarse scanning step increment ranges from approximately 50 microns to 200 microns.
5. The method of claim 1 , wherein a fine scanning step increment ranges from approximately 5 microns to 50 microns.
6. The method of claim 1 , wherein the step of coarsely scanning includes moving the microfluidic chip relative to a radiation source.
7. The method of claim 1 , wherein the step of coarsely scanning includes scanning the microfluidic chip across a plurality of microfluidic channels.
8. The method of claim 1 , wherein the microfluidic chip includes a plurality of pinholes associated one or more microfluidic channels and the step of coarsely scanning includes scanning the pinholes associated with the one or more microfluidic channels.
9. The method of claim 1 , further comprising:
determining a first-direction coarse misalignment distance based on the coarse scanning; and
translating the microfluidic chip by the negative of the first-direction coarse misalignment position.
10. The method of claim 9 , further comprising:
determining a first-direction fine misalignment distance based on the fine scanning; and
translating the microfluidic chip by the negative of the first-direction fine misalignment position.