Apparatus, methods and composition for synthesis of cannabinoid compounds
The disclosure provides systems and methods for producing a cannabinoid product, which comprises contacting a cannabinoid precursor in a first phase with a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible. The disclosure also provides a composition comprising the cannabinoid precursor in a first phase and a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible.
1. A kit for producing a cannabinoid or its analog, comprising a bioreactor containing a composition comprising a cannabinoid precursor in a first phase and a cannabinoid synthase in a second phase, wherein the first phase comprises an organic solvent and the second phase comprises an aqueous solvent.
2. The kit of claim 1 , further comprising a control mechanism configured to control at least one of pH, solvent, temperature, pressure, and flow rate of the composition in the bioreactor to modify the amount of a first cannabinoid relative to the amount of a second cannabinoid or its analog,
wherein the control mechanism comprises a processing circuit having a processor and a memory device.
3. The kit of claim 1 , wherein the cannabinoid precursor is CBGA, CBGVA, or their derivative or analog.
4. The kit of claim 1 , wherein the cannabinoid synthase comprises CBDA synthase or THCA synthase.
5. The kit of claim 1 , wherein the organic solvent comprises one or more of olive oil, sesame oil, castor oil, cotton-seed oil, soybean oil, butane, pentane, heptane, octane, isooctane, nonane, decane, and terpene.
6. The kit of claim 1 , wherein the volume ratio of the first phase to the second phase is from about 1:9 to about 9:1.
7. The kit of claim 1 , wherein the cannabinoid so produced comprises
(a) THCVA and CBCVA,
(b) CBDVA and CBCVA,
(c) THCA and CBCA, or
(d) CBDA and CBCA.