Microscope illumination device and microscope
A microscope illumination device includes a white LED light source and an illumination optical system. The white LED light source includes a substrate, a plurality of LED chips, and a fluorescent substance layer provided to cover the plurality of LED chips, the plurality of LED chips being arrayed on the substrate and being configured to emit excitation light. The illumination optical system includes a field stop and a light diffusion element that is arranged between the white LED light source and the field stop. The microscope illumination device satisfies 0.2< d/p <1 (1) where p is a minimum interval between centers of the plurality of LED chips and d is a size of each of the plurality of LED chips.
1. A microscope illumination device comprising:
a white LED light source including a substrate, a plurality of LED chips, and a fluorescent substance layer provided to cover the plurality of LED chips, the plurality of LED chips being arrayed on the substrate and being configured to emit excitation light; and
an illumination optical system that (i) irradiates a subject with light from the white LED light source, (ii) includes a field stop and a light diffusion element, and (iii) is arranged to illuminate the subject by Kohler illumination, the light diffusion element being arranged between the white LED light source and the field stop and being configured to diffuse the light,
wherein the illumination optical system further includes:
a collector lens that is arranged between the white LED light source and the light diffusion element;
an aperture stop that is arranged in such a manner that the field stop is positioned between the light diffusion element and the aperture stop;
a relay lens that is arranged between the field stop and the aperture stop; and
a condenser lens that is arranged in such a manner that the aperture stop is positioned between the relay lens and the condenser lens, and
wherein conditional expressions of:
0.2< d/p< 1 (1)
( p−d )/(2× F co )≤NA1× F cd /F r1 (2)
and
NA1/NA<⅓ (3)
are satisfied, where p is a minimum interval between centers of the plurality of LED chips, d is a size of each of the plurality of LED chips, F co is a focal length of the collector lens, F rl is a focal length of the relay lens, F cd is a focal length of the condenser lens, NA is a numerical aperture of the condenser lens, and NA1 is a numerical aperture of an objective with a magnification corresponding to a maximum illumination scope of the condenser lens.
2. The microscope illumination device according to claim 1 , further satisfying a conditional expression of:
½×tan −1 (( p−d )/ F co )≤θ≤6×tan −1 (( p−d )/ F co ) (4)
where θ is a scattering angle of diffused light emitted from the light diffusion element, the scattering angle being an angle at which the diffused light is emitted from the light diffusion element with an intensity that is ½ of that of diffused light emitted in a traveling direction of entering light.
3. The microscope illumination device according to claim 2 , further satisfying a conditional expression of:
NA× F cd /F rl <R /(2× F co )+sin θ (5)
where R is a diameter of a minimum circle that includes the plurality of LED chips.
4. The microscope illumination device according to claim 3 , further satisfying a conditional expression of:
0.5≤ R/φ LED (6)
where φ LED is a diameter of a circle that is inscribed in the fluorescent substance layer.
5. The microscope illumination device according to claim 1 , further satisfying a conditional expression of:
0.5≤ R/φ LED (6)
where φ LED is a diameter of a circle that is inscribed in the fluorescent substance layer, and R is a diameter of a minimum circle that includes the plurality of LED chips.
6. The microscope illumination device according to claim 2 , further satisfying a conditional expression of:
0.5≤ R/φ LED (6)
where φ LED is a diameter of a circle that is inscribed in the fluorescent substance layer and R is a diameter of a minimum circle that includes the plurality of LED chips.
7. The microscope illumination device according to claim 1 , further satisfying a conditional expression of:
NA× F cd /F rl <R /(2× F co )+sin θ (5)
where θ is a scattering angle of diffused light emitted from the light diffusion element, the scattering angle being an angle at which the diffused light is emitted from the light diffusion element with an intensity that is ½ of that of diffused light emitted in a traveling direction of entering light and R is a diameter of a minimum circle that includes the plurality of LED chips.
8. The microscope illumination device according to claim 7 , further satisfying a conditional expression of:
0.5≤ R/φ LED (6)
where φ LED is a diameter of a circle that is inscribed in the fluorescent substance layer.
9. A microscope illumination device comprising:
a white LED light source including a substrate, a plurality of LED chips, and a fluorescent substance layer provided to cover the plurality of LED chips, the plurality of LED chips being arrayed on the substrate and being configured to emit excitation light; and
an illumination optical system that (i) irradiates a subject with light from the white LED light source, (ii) includes a field stop and a light diffusion element, and (iii) is arranged to illuminate the subject by Kohler illumination, the light diffusion element being arranged between the white LED light source and the field stop and being configured to diffuse the light,
wherein the illumination optical system further includes a collector lens that is arranged between the white LED light source and the light diffusion element, and
wherein conditional expressions of:
0.2< d/p< 1 (1) and
½×tan −1 (( p−d )/ F co )≤θ≤6×tan −1 (( p−d )/ F co ) (4)
are satisfied, where p is a minimum interval between centers of the plurality of LED chips, d is a size of each of the plurality of LED chips, F co is a focal length of the collector lens, and θ is a scattering angle of diffused light emitted from the light diffusion element, the scattering angle being an angle at which the diffused light is emitted from the diffusion element with an intensity that is ½ of that of diffused light emitted in a traveling direction of entering light.
10. The microscope illumination device according to claim 9 ,
wherein the illumination optical system further includes:
an aperture stop that is arranged in such a manner that the field stop is positioned between the light diffusion element and the aperture stop;
a relay lens that is arranged between the field stop and the aperture stop; and
a condenser lens that is arranged in such a manner that the aperture stop is positioned between the relay lens and the condenser lens, and
wherein a conditional expression of:
NA× F cd /F rl <R /(2× F co )+sin θ (5)
is satisfied, where F rl is a focal length of the relay lens, F cd is a focal length of the condenser lens, NA is a numerical aperture of the condenser lens, and R is a diameter of a minimum circle that includes the plurality of LED chips.
11. The microscope illumination device according to claim 10 , further satisfying a conditional expression of:
0.5≤ R/φ LED (6)
where φ LED is a diameter of a circle that is inscribed in the fluorescent substance layer.
12. The microscope illumination device according to claim 9 , further satisfying a conditional expression of:
0.5≤ R/φ LED (6)
where φ LED is a diameter of a circle that is inscribed in the fluorescent substance layer, and R is a diameter of a minimum circle that includes the plurality of LED chips.
13. A microscope illumination device comprising:
a white LED light source including a substrate, a plurality of LED chips, and a fluorescent substance layer provided to cover the plurality of LED chips, the plurality of LED chips being arrayed on the substrate and being configured to emit excitation light; and
an illumination optical system that (i) irradiates a subject with light from the white LED light source, (ii) includes a field stop and a light diffusion element, and (iii) is arranged to illuminate the subject by Kohler illumination, the light diffusion element being arranged between the white LED light source and the field stop and being configured to diffuse the light,
wherein the illumination optical system further comprises:
a collector lens that is arranged between the white LED light source and the light diffusion element;
an aperture stop that is arranged in such a manner that the field stop is positioned between the light diffusion element and the aperture stop;
a relay lens that is arranged between the field stop and the aperture stop; and
a condenser lens that is arranged in such a manner that the aperture stop is positioned between the relay lens and the condenser lens, and
wherein conditional expressions of:
0.2< d/p< 1 (1) and
NA× F cd /F rl <R /(2× F co )+sin θ (5)
are satisfied, where p is a minimum interval between centers of the plurality of LED chips, d is a size of each of the plurality of LED chips, F co is a focal length of the collector lens, F rl is a focal length of the relay lens, F cd is a focal length of the condenser lens, NA is a numerical aperture of the condenser lens, and θ is a scattering angle of diffused light emitted from the light diffusion element, the scattering angle being an angle at which the diffused light is emitted from the diffusion element with an intensity that is ½ of that of diffused light emitted in a traveling direction of entering light, and R is a diameter of a minimum circle that includes the plurality of LED chips.
14. The microscope illumination device according to claim 13 , further satisfying a conditional expression of:
0.5≤ R/φ LED (6)
where φ LED is a diameter of a circle that is inscribed in the fluorescent substance layer.