Method and apparatus for isolating nucleic acids
A method comprises: sorbing a sample solution comprising nucleic acids to a sample receiving portion of a quartz fiber filter by contacting the sample solution with the sample receiving portion; and washing the sample receiving portion while keeping most of nucleic acids around the sample receiving portion by flowing a wash solution through the sample receiving portion under a wicking force directed away from the sample receiving portion. An associated apparatus is also provided.
1. An apparatus, comprising:
a quartz fiber filter comprising:
a sample receiving portion for sorbing a sample solution comprising nucleic acids by contacting with the sample solution and keeping most of nucleic acids around the sample receiving portion after a wash solution flowing therethrough under a wicking force directed away from the sample receiving portion;
wherein the quartz fiber filter comprises a wash portion spatially distant from the sample receiving portion; and
wherein the quartz fiber filter is an elongated strip comprising a first end, the wash portion, the sample receiving portion, and a second end, the wash portion is located closer to the first end than to the second end and the sample receiving portion is located closer to the second end than the wash portion; and
a housing enclosing the quartz fiber filter therein, wherein the housing comprises a sample opening exposing the sample receiving portion and a wash opening spatially distant from the sample opening, exposing the wash portion of the filter.
2. The apparatus of claim 1 , wherein the quartz fiber filter has a particle retention efficiency of about 98% for particles of a size of no less than 2.2 μm, a basis weight of 85 g/m 2 , and a thickness in a range of from about 300 μm to about 600 μm.
3. The apparatus of claim 1 , further comprising: a bibulous pad made of at least one of cellulose, silica microfiber filter, glass fiber, and quartz filter, and located adjacent to and vertically aligned with at least one of the wash portion and the second end.
4. The apparatus of claim 1 , wherein the wash portion is the first end.
5. The apparatus of claim 1 , further comprising: a bibulous pad made of at least one of cellulose, silica microfiber filter, glass fiber, and quartz filter and adjacent to and vertically aligned with the quartz fiber filter.
6. The apparatus of claim 1 , wherein the sample solution comprises chaotropic salt and the washing solution comprises at least one of ethanol and isopropanol.
7. The apparatus of claim 1 , wherein the housing further comprises a wash opening exposing the wash portion of the quartz fiber filter.
8. The apparatus of claim 7 , wherein the housing comprises an upper half and a lower half wherein the sample opening and the wash opening are located in the upper half of the housing.