Microscopy system, microscopy method, and computer readable recording medium
View Patent ↗A microscopy system includes: an imaging unit configured to capture a subject image generated by an observation optical system of a microscope, and acquire an image; a shifting unit configured to shift positions of a focal plane and a field of view of the observation optical system; an imaging control unit configured to cause the imaging unit to acquire a multi-focused image by shifting the positions of the focal plane and the field of view in one exposure period of the imaging unit; a shift amount acquisition processing unit configured to acquire a shift amount, by which the position of the field of view is shifted; an all-focused image generating unit configured to respectively generate plural all-focused images based on plural multi-focused images and on blurring information; and a display unit configured to consecutively display the generated plural all-focused images.
1. A microscopy system comprising:
a processor comprising hardware, wherein the processor is configured to:
control an image sensor to acquire N*M multi-focused images of a subject captured by using an observation optical system of a microscope,
wherein N represents the number of depth of field and each N of the N*M multi-focused images is acquired by shifting both of positions of a focal plane and a field of view of the observation optical system, and
wherein shift amounts of the field of view are different among M of the N*M multi-focused images; and
generate M all-focused images,
wherein each of the M all-focused images is based on N of the N*M multi-focused images; and
control a display to consecutively display the M all-focused images generated,
wherein each of N and M is a natural number.
2. The microscopy system according to claim 1 ,
wherein the processor is configured to determine an imaging start position for acquisition of each of the N*M multi-focused images, based on the shift amount acquired.
3. The microscopy system according to claim 1 ,
wherein the processor is further configured to:
determine, as an observed region, a region selected from any one of the M all-focused images, according to an operation performed from outside; and
extract regions corresponding to the observed region from the remaining all-focused images of the M all-focused images other than the any one of the M all-focused images, from which the observed region has been selected, and acquire, based on shift amounts between a position of the observed region in the any one of the M all-focused images, from which the observed region has been selected, and positions of the regions in the M all-focused images, from which the regions have been extracted, a position of a slice including a structure in a subject, the structure corresponding to the observed region.
4. The microscopy system according to claim 1 ,
wherein the each of the M all-focused images is based on N of the N*M multi-focused images, and on blurring information of images according to the shift amounts, and
wherein the blurring information is information generated by use of the shift amounts, and point spread functions representing blurring in the images.
5. The microscopy system according to claim 4 ,
wherein the processor is configured to cause the image sensor to acquire, at the same shift amount, image information in each of the N of the N*M multi-focused images of the each of the M all-focused images.
6. A microscopy method comprising:
controlling an image sensor to acquire N*M multi-focused images of a subject captured by using an observation optical system of a microscope,
wherein N represents the number of depth of field and each N of the N*M multi-focused images is acquired by shifting both of positions of a focal plane and a field of view of the observation optical system, and
wherein shift amounts of the field of view are different among M of the N*M multi-focused images; and
generating M all-focused images,
wherein each of the M all-focused images is based on N of the N*M multi-focused images; and
controlling a display to consecutively display the M all-focused images generated,
wherein each of N and M is a natural number.
7. A non-transitory computer-readable recording medium on which an executable program is recorded, wherein the program causes a processor to at least perform:
controlling an image sensor to acquire N*M multi-focused images of a subject captured by using an observation optical system of a microscope,
wherein N represents the number of depth of field and each N of the multi-focused images is acquired by shifting both of positions of a focal plane and a field of view of the observation optical system, and
wherein shift amounts of the field of view are different among M of the N*M multi-focused images;
generating M all-focused images, wherein each of the M all-focused images is based on N of the N*M multi-focused images; and
controlling a display to display displaying consecutively the M plural all-focused images,
wherein each of N and M is a natural number.