Methods for raising antibodies
The present invention generally relates to methods of generating antibodies against a species of pathogen that involve identifying the pathogen that is most genetically representative of member of pathogen species and using the identified pathogen to generate an antibody.
1. A method of generating antibodies against a species comprising pathogens, the method comprising:
obtaining a nucleic acid from a plurality of pathogens within the species;
assaying said nucleic acids to determine genetic similarities between the pathogens, wherein assaying comprises assessing the level of similarity by an analytical method selected from the group consisting of ribotyping, rep-PCR, pulsed field gel electrophoresis, optical mapping, and microarray-based measurement of single nucleotide polymorphisms;
separating the pathogens into at least two different groups based upon the genetic similarities;
isolating at least one, but not all, pathogen from each group that exhibits representative characteristics of the group to form a subset of pathogens; and
generating antibodies for each isolated pathogen by administering each isolated pathogen to one or more mammals in an amount effective to produce an immune response in order to produce a set of antibodies.
2. The method of claim 1 , wherein the nucleic acid is DNA.
3. The method of claim 1 , wherein the pathogen comprises bacteria.
4. The method of claim 3 , wherein the bacteria comprise bacteria of the same species but of different serotypes.
5. The method of claim 3 , wherein the bacteria comprise bacteria of the same species but of different strains.
6. The method of claim 1 , wherein assaying said nucleic acids comprises sequencing a portion of said nucleic acids and comparing the sequences.
7. The method of claim 1 , wherein administering comprises injecting a mammal with each isolated pathogen.
8. The method of claim 7 , wherein generating an antibody further comprises placing the pathogen in adjuvant prior to injecting the mammal.
9. The method of claim 1 , wherein the antibodies are monoclonal antibodies.
10. The method of claim 1 , wherein the antibodies are polyclonal antibodies.
11. The method of claim 1 , wherein isolating the at least one pathogen comprises building a cladogram where genomically similar pathogens are grouped together to form clades.
12. The method of claim 1 , wherein the subset of pathogens is representative of the genomic variation present in the species of pathogen.
13. The method of claim 1 , wherein the nucleic acid is RNA.