PERICYTE CELL EXOSOMES
Compositions and methods of use pertaining to exosomes, and more particularly to exosomes from pericytes and endothelial progenitor cells are presented.
1 . A composition comprising, exosomes isolated from a pericyte-like cell line, wherein the pericyte-like cell line is derived from pluripotent stem cells and wherein the exosomes are capable of one or both of stimulating or stabilizing the formation of vascular tube networks.
2 . The composition of claim 1 , wherein the exosomes are nonimmunogenic.
3 . The composition of claim 2 , wherein the exosomes display only background levels of one or more of MHC I and MHC II antigens.
4 . The composition of claim 1 , wherein the exosomes are isolated from self-renewing perivascular progenitor cells derived from embryonic stem cells.
5 . The composition of claim 1 , wherein the stem cells are human embryonic stem cells (hESC) or induced pluripotent stem cells.
6 . The composition of claim 1 , wherein when the exosomes are capable of retaining vascular tube networks by between about 20% to about 100%.
7 . The composition of claim 1 , wherein when the exosomes are capable of retaining vascular tube networks by at least about 73%.
8 . The composition of claim 1 , wherein the exosomes are administered to a subject such that the exosomes come into contact with the subject's vasculature.
9 . The composition of claim 8 , wherein the exosomes are at a concentration of between about 1,000,000 particles/μl to about 10,000,000 particles/μl.
10 . The composition of claim 1 , further comprising exosomes isolated from endothelial cell lines.
11 . The composition of claim 1 , wherein the exosomes enhance vascular tube formation by at least about 30%, at least about at least about 35%, at least about 40%, at least about 50%, at least about 55%, at least about 60%, at least about 75%, at least about 100%, about 20% to about 150%.
12 . The composition of claim 11 , wherein the enhancement of vascular tube formation is in comparison to exosomes isolated from other cell types.
13 . The composition of claim 1 , wherein the exosomes express one or more of the markers CD146, CD105, and CD73 but only minimal levels of the markers CD133, CD144, and CD31.
14 . The composition of claim 1 , wherein the exosomes are capable of stabilizing the formation of vascular tube networks for at least about 1 week.
15 . A method for treating a vascular disease, disorder, or traumatic injury in a subject comprising, administering to the subject a composition comprising exosomes isolated from a pericyte-like cell line, wherein the pericyte-like cell line is derived from pluripotent stem cells and wherein the exosomes are capable of one or both of stimulating or stabilizing the formation of vascular tube networks.
16 . The method of claim 15 , wherein the exosomes are administered to the subject such that they come into contact with the subject's vasculature.
17 . The method of claim 15 , wherein the exosomes enhance vascular tube formation by at least about 30%, at least about at least about 35%, at least about 40%, at least about 50%, at least about 55%, at least about 60%, at least about 75%, at least about 100%, about 20% to about 150%.
18 . The method of claim 17 , wherein the enhancement of vascular tube formation is in comparison to exosomes isolated from other cell types.
19 . The method of claim 15 , wherein the exosomes are at a concentration of between about 1,000,000 particles/μl to about 10,000,000 particles/μl.
20 . The composition of claim 15 , wherein the exosomes are nonimmunogenic.
21 . The composition of claim 20 , wherein the exosomes display only background levels of one or more of MHC I and MHC II antigens.
22 . The composition of claim 15 , wherein the exosomes are isolated from self-renewing perivascular progenitor cells derived from embryonic stem cells.
23 . The composition of claim 15 , wherein the stem cells are human embryonic stem cells (hESC) or induced pluripotent stem cells.
24 . The composition of claim 15 , wherein when the exosomes are capable of retaining vascular tube networks by between about 20% to about 100%.
25 . The composition of claim 15 , wherein when the exosomes are capable of retaining vascular tube networks by at least about 73%.