Genetic markers associated with drought tolerance in maize
The presently disclosed subject matter relates to methods and compositions for identifying, selecting, and/or producing drought tolerant maize plants or germplasm. Maize plants or germplasm that have been identified, selected, and/or produced by any of the methods of the presently disclosed subject matter are also provided.
1. A method of producing a maize plant having improved water optimization, the method comprising the steps of:
a) isolating at least one nucleic acid from a maize plant selected from a population of maize plants;
b) detecting by way of amplifying a marker locus or a portion of a marker locus from the nucleic acid of a) and thereby, producing a amplicon diagnostic for at least one allele located within 10 cM to a water optimization locus that is associated with increased water optimization, wherein the water optimization locus comprises the maize genomic region ZmDhn1, and further wherein ZmDhn1 comprises the following alleles corresponding to SEQ ID NO: 36 comprising a C nucleotide at the position that corresponds to position 500 of SEQ ID NO: 36, a G nucleotide at the position that corresponds to position 568 of SEQ ID NO: 36, and a T nucleotide at the position that corresponds to position 698 of SEQ ID NO: 36 on chromosome 6 in the first plant's genome;
c) selecting a first maize plant from the population of a) based on the presence of the water optimization locus in b);
d) crossing the first maize plant with a second maize plant, wherein the second maize plant does not have in its genome the water optimization locus or allele detected in b); and
e) producing a progeny maize plant having improved water optimization from the cross of d) wherein said progeny plant comprises the water optimization locus as well as the allele detected in b).
2. The method of claim 1 , wherein the second maize plant or progeny maize plant in e) is backcrossed one or more times.
3. The method of claim 1 , wherein the water optimization locus is on Zea mays chromosome 6.
4. The method of claim 1 , wherein the genomic region consist of SEQ ID NO: 36.
5. The method of claim 1 , wherein either the first maize plant or second maize plant is a inbred.
6. The method of claim 1 , wherein the water optimization locus comprises haplotype I.
7. The method of claim 1 , wherein the nucleotide probe or primer used to detect at least one allele within a water optimization locus that is associated with increased water optimization in the first maize plant comprises SEQ ID NO: 95.
8. A maize plant having introgressed into its genome at least one allele detected in the maize parental line within a water optimization locus that is associated with increased water optimization, wherein the water optimization locus comprises the Zea maize genomic region ZmDhn1 and, and further wherein ZmDhn1 comprises the following alleles corresponding to SEQ ID NO: 36 comprising a C nucleotide at the position that corresponds to position 500 of SEQ ID NO: 36, a G nucleotide at the position that corresponds to position 568 of SEQ ID NO: 36, and a T nucleotide at the position that corresponds to position 698 of SEQ ID NO: 36 on chromosome 6 in the first plant's genome.
9. The Zea maize plant of claim 8 , wherein the water optimization locus is located on Zea mays chromosome 6.
10. The maize plant of claim 8 , wherein the maize genomic region consists of SEQ ID NO: 95.
11. The maize plant of claim 8 , wherein the maize plant is an elite maize plant.
12. The maize plant of claim 8 , wherein the water optimization locus comprises haplotype I.