Method for processing the contents of a processing vial within an instrument
View Patent ↗One or more temperature cycles are applied to the contents of a processing vial closed by an interlocked cap within a thermal cycler. The processing vial and the interlocked cap are transferred from the thermal cycler to a storage compartment within the instrument with a transfer mechanism with a probe engaged with the cap. In the storage compartment, the processing vial and interlocked cap are moved to an access opening in the instrument and are removed from the instrument through the access opening.
1. A method for processing the contents of a processing vial within an instrument, the method comprising:
(A) applying one or more temperature cycles to the contents of the processing vial within a thermal cycler, the processing vial being closed by a cap that is interlocked to the processing vial;
(B) engaging the cap with a probe of a transfer mechanism inserted into an open top end of the cap;
(C) with the transfer mechanism, transferring the processing vial and the interlocked cap engaged with the probe from the thermal cycler to a storage compartment within the instrument;
(D) within the storage compartment, moving the processing vial and interlocked cap to an access opening in the instrument; and
(E) removing the processing vial and interlocked cap from the instrument through the access opening.
2. The method of claim 1 , wherein the access opening comprises a drawer in an exterior panel of the instrument.
3. The method of claim 1 , wherein the storage compartment comprises a well plate, and step (C) comprises placing the processing vial and the interlocked cap in the well plate.
4. The method of claim 3 , wherein step (D) comprises translating the well plate with a drive system comprising a motor-driven belt operably coupled to the well plate.
5. The method of claim 1 , wherein the transfer mechanism comprises a robotic pipettor.
6. The method of claim 1 , wherein the cap is interlocked to the processing vial by a latch collar extending about the cap engaged with latch hooks formed on the processing vial.
7. The method of claim 1 , wherein the cap is interlocked to the processing vial by latch fingers of the cap lockingly engaged with a lock collar of the processing vial.
8. The method of claim 1 , further comprising processing the processing vial in a centrifuge prior to step (A).
9. The method of claim 1 , wherein step (E) comprises manually removing the processing vial and interlocked cap from the instrument through the access opening.
10. The method of claim 1 , wherein step (A) further comprises measuring optical signals from the contents of the processing vial during each temperature cycle.
11. The method of claim 10 , wherein the optical signal comprises fluorescence emitted from the contents of the processing vial.
12. The method of claim 11 , further comprising detecting fluorescence signals at one or more wavelengths, and the measuring step comprises periodically measuring fluorescence intensity at each wavelength at regular intervals.
13. The method of claim 1 , wherein the one or more temperature cycles comprise temperature cycles for performing a Polymerase Chain Reaction.
14. The method of claim 3 , wherein the well plate comprises a 96 well plate.