Compositions and methods for treating joints

1. A medical kit, comprising:

a syringe including a first chamber, a second chamber that is separate from the first chamber, and a plunger, wherein the first and second chambers are separated from each other by a valve;

a first component disposed within the first chamber, the first component comprising hyaluronic acid having a molecular weight of about 1.0 MDa to about 5.0 MDa; and

a second component disposed within the second chamber, the second component consisting essentially of a lyophilized glycosaminoglycan, wherein the lyophilized glycosaminoglycan comprises unmodified chondroitin sulfate;

wherein the plunger is configured to move, relative to the valve, to move the first component into the second chamber so as to combine the first and second components and thereby form a mixture having a viscosity suitable for injection through the syringe for a predetermined period of time.

2. The kit of claim 1 , further comprising a needle that is configured to be coupled to a distal end of the syringe;

wherein the needle is configured to inject the mixture into tissue during the predetermined period of time.

3. The kit of claim 1 , wherein the lyophilized glycosaminoglycan comprises chondroitin sulfate.

4. The kit of claim 1 , further comprising a third component disposed within the second chamber, the third component comprising glucosamine.

5. The kit of claim 1 , wherein the predetermined period of time is in a range of about 1 minute to about 60 minutes.

6. The kit of claim 1 , wherein the syringe includes a housing with a sidewall that defines the first and second chambers, the movement of the plunger to move the first component into the second chamber comprises movement of the plunger within the first chamber and the valve is seated in the housing with an outer perimeter of the valve abutting an inner surface of the sidewall along an entire circumference of the inner surface.

7. A medical method, comprising:

mixing hyaluronic acid and a composition consisting essentially of unmodified lyophilized glycosaminoglycan to form a mixture, wherein the hyaluronic acid has a molecular weight of about 1.0 MDa to 5.0 MDa, and wherein the hyaluronic acid is present in the mixture at a concentration between about 7 mg/ml and about 60 mg/ml by weight of the mixture; and

injecting the mixture into tissue within a predetermined period of time after the mixing, wherein the mixture, during the predetermined period of time, has a viscosity that is less than a viscosity of the hyaluronic acid prior to the mixing;

wherein, prior to the mixing, the hyaluronic acid is disposed within a first chamber of a syringe;

wherein the mixing of the hyaluronic acid and the unmodified lyophilized glycosaminoglycan comprises moving the hyaluronic acid from a first chamber of a syringe to a second chamber of the syringe that is separate from the first chamber; and

wherein, prior to the mixing, the second chamber has the unmodified lyophilized glycosaminoglycan disposed therein.

8. A medical method, comprising:

actuating at room temperature the plunger of the kit of claim 1 , thereby injecting the first component into the second chamber and combining the first and second components.

9. The method of claim 8 , wherein the mixture has a second viscosity after the predetermined period of time, the second viscosity being greater than the viscosity.

10. The method of claim 9 , wherein the actuating occurs within the predetermined period of time after the formation of the mixture, and the predetermined period of time is in a range of about 1 minute to about 30 minutes.

11. The kit of claim 1 , wherein the valve has a disc shape.

12. The method of claim 8 , wherein the first component disposed within the first chamber has a viscosity, and during the predetermined period of time the viscosity of the mixture is less than the viscosity of the first component disposed within the first chamber.

13. The method of claim 8 , wherein the first and second components are present within the mixture at a ratio of first component to second component in the range of about 1:0.005 to about 1:100.

14. The method of claim 8 , wherein the hyaluronic acid is present in the mixture at a concentration between about 7 mg/ml and about 60 mg/ml by weight of the mixture.

15. The method of claim 8 , wherein the actuating occurs within the predetermined period of time after the formation of the mixture, and predetermined period of time is in a range of about 1 minute to about 60 minutes.

16. The method of claim 7 , wherein the period of time is about 30 minutes.

17. The method of claim 7 , wherein the lyophilized glycosaminoglycan comprises chondroitin sulfate.

18. The method of claim 7 , wherein the hyaluronic acid and the lyophilized glycosaminoglycan are mixed at room temperature.

19. The method of claim 7 , wherein the period of time is in a range of about 1 minute to about 60 minutes.

20. The method of claim 9 , wherein the first and second components are present within the mixture at a ratio of first component to second component in the range of about 1:0.005 to about 1:100; and

the hyaluronic acid is present in the mixture at a concentration between about 7 mg/ml and about 60 mg/ml by weight of the mixture.

21. The method of claim 7 , wherein moving the hyaluronic acid from the first chamber of the syringe to the second chamber of the syringe comprises moving, relative to a valve of the syringe that separates the first and second chambers, a plunger of the syringe within the first chamber.