Methods and apparatus for improved sample visualization
Methods and devices are provided for use with a biological sample, wherein in one example, the device comprising a sample holder and an excitation source for providing excitation energy into the light conduit towards the sample holder. In one embodiment, the device comprises an imaging cytometer. Optionally, the device comprises a flow cytometer. Optionally, a light conduit comprises an optical fiber. Optionally, the optical fiber has an outer cross-sectional shape different from an inner optical core cross-sectional shape.
1. A method comprising:
measuring chamber height of a cuvette;
encoding a measurement of said chamber height in a data storage unit on the cuvette;
using said measurement when said cuvette is used in a sample processing device to correct for variability in cuvette chamber dimensions; and
illuminating a sample chamber of a cuvette with a plurality of light sources, each of said light sources optically coupled to the sample chamber by an optical conduit with an outer cross-sectional shape different from an inner optical core cross-sectional shape that is of the same shape as the sample chamber, wherein the optical conduit is directed to a collimation lens, a color filter, and a focusing lens towards the sample chamber to provide illumination with a non-Gaussian intensity profile.
2. The method of claim 1 wherein the sample chamber has a rectangular shape when viewed top-down and the inner optical core cross-sectional shape is also rectangular.
3. The method of claim 1 wherein the cuvette further comprises a bar code thereon.
4. The method of claim 1 wherein the light sources comprises diode lasers.
5. The method of claim 1 wherein the cuvette is holds the sample for an imaging cytometer.
6. The method of claim 1 wherein the cuvette holds the sample for a flowing cytometer.