Bioactive polypeptides for improvements in plant protection, growth and productivity
Bioactive priming polypeptides are provided that are useful when applied to plants in agricultural formulations. Methods of using the formulations containing the bioactive priming polypeptides are also provided which are applied exogenously to the surface of a plant or a plant cell membrane or endogenously to the interior of a plant or to a plant cell. The bioactive priming polypeptides when applied to a plant, a plant part, or a plant growth medium or a rhizosphere in an area surrounding the plant or the plant part increase growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part and/or decrease abiotic stress in the plant or the plant part and/or protect the plant or the plant part from disease, insects and/or nematodes, and/or increase the innate immune response of the plant or the plant part and/or change plant architecture.
1. An isolated peptide for bioactive priming of a plant or a plant part to increase growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part and/or decrease abiotic stress in the plant or the plant part and/or protect the plant or the plant part from disease, insects and/or nematodes, and/or increase the innate immune response of the plant or the plant part and/or change plant architecture, wherein the peptide comprises a flagellin or flagellin-associated peptide having a length of 22 or fewer amino acids, wherein the amino acid sequence of the flagellin or flagellin-associated peptide comprises any one of SEQ ID NOs: 526, 528, 532, 534, 571, 586, 754-759, and 761-765.
2. The peptide of claim 1 , wherein the flagellin or flagellin-associated peptide: contains a chemical modification; is part of a fusion protein wherein the fusion protein-contains a protease recognition sequence; or a combination thereof.
3. The peptide of claim 2 , wherein the chemical modification comprises acetylation, amidation, cyclization, or PEGylation.
4. The peptide of claim 1 , wherein the amino acid sequence of the peptide consists of 22 amino acids.
5. The peptide of claim 1 , wherein the peptide is part of a fusion protein and the fusion protein further comprises an assistance peptide.
6. The peptide of claim 5 , wherein the assistance peptide comprises:
a signature peptide, and an amino acid sequence of the signature peptide comprises any one of SEQ ID NOs: 542-548, or any combination thereof; or
a signal anchor sorting peptide, and an amino acid sequence of the signal anchor sorting peptide comprises any one of SEQ ID NOs: 549-562, or any combination thereof.
7. The peptide of claim 1 , wherein the peptide comprises the amino acid sequence of any one of SEQ ID NOs: 226-229, 232-234, 236-240, 243-246, 248, 250-256, 258-259, 261, 263, 265-270, 272-280, 282-283, 285-286, 288-300, 526, 528, 532, 534, 536, 538, 540, 571-586, and 752.
8. The peptide of claim 1 , wherein the peptide is chemically synthesized, concentrated from a fermentation product, and/or purified, by filtration, chromatography, or from a recombinant microorganism.
9. A composition for bioactive priming of a plant or a plant part to increase growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part and/or decrease abiotic stress in the plant or the plant part and/or protect the plant or the plant part from disease, insects and/or nematodes, and/or increase the innate immune response of the plant or the plant part and/or change plant architecture, the composition comprising the peptide of claim 1 and an agrochemical or a carrier.
10. A seed coated with the peptide of claim 1 , a composition comprising a peptide of claim 1 and a carrier or agrochemical, or a recombinant microorganism expressing or overexpressing at least one peptide of claim 1 .
11. The peptide of claim 1 , wherein the amino acid sequence of the peptide comprises SEQ ID NO: 226.
12. The peptide of claim 1 , wherein the amino acid sequence of the peptide consists essentially of SEQ ID NO: 226.
13. The peptide of claim 1 , wherein the amino acid sequence of the peptide consists of SEQ ID NO: 226.
14. The peptide of claim 2 , wherein the peptide comprises the amino acid sequence of any one of SEQ ID NOs: 226-229, 232-234, 236-240, 243-246, 248, 250-256, 258-259, 261, 263, 265-270, 272-280, 282-283, 285-286, 288-300, 526, 528, 532, 534, 536, 538, 540, 571-586, and 752.
15. The peptide of claim 1 wherein the amino acid sequence of the peptide consists of 15 amino acids.