Tissue-specific expression and hybrid plant production
This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in male plant tissues, for example, to provide an economical male sterility system of hybrid seed production.
1. A nucleic acid molecule comprising a coding sequence operably linked to a plant promoter, wherein the coding sequence comprises the internal small ribonucleic acid (sRNA) target site of SEQ ID NO:7.
2. The nucleic acid molecule of claim 1 , wherein the molecule is a vector.
3. The nucleic acid molecule of claim 2 , wherein the vector is a plant expression vector or plant transformation vector.
4. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule is incorporated into a plant genomic nucleic acid.
5. The nucleic acid molecule of claim 1 , wherein the coding sequence is an herbicide tolerance coding sequence or a selectable marker coding sequence.
6. The nucleic acid molecule of claim 1 , wherein the plant promoter is sugarcane bacilliform virus (SCBV) promoter.
7. A transgenic plant cell comprising the nucleic acid molecule of claim 1 .
8. The transgenic plant cell of claim 7 , wherein expression of the coding sequence is reduced or eliminated in the transgenic plant cell.
9. The transgenic plant cell of claim 7 , wherein expression of the coding sequence is reduced or eliminated in a plurality of transgenic plant cells, thereby resulting in reduced or eliminated expression of the coding sequence in a tissue-specific or tissue-preferred manner.
10. The transgenic plant cell of claim 9 , wherein expression of the coding sequence is reduced or eliminated in a plurality of transgenic plant cells, thereby resulting in reduced or eliminated expression of the coding sequence in male plant tissues.
11. A transgenic plant tissue or plant part comprising the nucleic acid molecule of claim 1 .
12. The transgenic plant tissue or plant part of claim 11 , wherein expression of the coding sequence is reduced or eliminated in male plant tissues.
13. The transgenic plant tissue or plant part of claim 11 , wherein the plant tissue or plant part is a seed.
14. A transgenic plant comprising the nucleic acid molecule of claim 4 .
15. The transgenic plant of claim 14 , wherein expression of the coding sequence is reduced or eliminated in at least one tissue of the plant.
16. The transgenic plant of claim 15 , wherein expression of the coding sequence is reduced or eliminated in a tissue-specific or tissue-preferred manner.
17. The transgenic plant of claim 16 , wherein expression of the coding sequence is reduced or eliminated in male plant tissues.
18. The transgenic plant of claim 17 , wherein the coding sequence is an herbicide tolerance coding sequence, and wherein application of the herbicide to the plant kills male tissues in the plant.
19. A method for producing a transgenic plant cell, the method comprising transforming a plant cell with the nucleic acid molecule of claim 1 .
20. The method according to claim 19 , the method further comprising:
culturing the transformed plant cell to produce a transgenic plant cell or transgenic tissue culture of transgenic plant cells comprising the coding sequence operably linked to the plant promoter; and
regenerating a transgenic plant from the transgenic plant cell or transgenic tissue culture.