Nucleic acid vaccines
View Patent ↗The invention relates to compositions and methods for the preparation, manufacture and therapeutic use ribonucleic acid vaccines (NAVs) comprising polynucleotide molecules encoding one or more antigens.
1. A method of vaccinating a subject comprising administering to the subject a nucleic acid vaccine comprising one or more messenger ribonucleic acid (mRNA) polynucleotides comprising an open reading frame encoding an antigenic polypeptide that is derived from an infectious agent, wherein the mRNA polynucleotide is not self-replicating RNA, wherein the mRNA polynucleotides are formulated within a cationic lipid nanoparticle having a molar ratio of about 20-60% ionizable cationic lipid: about 5-25% non-cationic lipid: about 25-55% sterol; and about 0.5-15% PEG-modified lipid, wherein the nucleic acid vaccine elicits an immune response having a longer lasting antibody titer than an antibody titer elicited by a reference nucleic acid vaccine comprising the one or more mRNA polynucleotides not formulated within a cationic lipid nanoparticle having a molar ratio of about 20-60% ionizable cationic lipid: about 5-25% non-cationic lipid: about 25-55% sterol; and about 0.5-15% PEG-modified lipid.
2. The method of claim 1 , wherein the method comprises administering to the subject a single dosage of between 0.001 mg/kg and 0.005 mg/kg of the nucleic acid vaccine in an effective amount to vaccinate the subject.
3. The method of claim 1 , wherein the open reading frame is codon-optimized.
4. The method of claim 1 , wherein the ionizable cationic lipid nanoparticle has a polydispersity value of less than 0.4.
5. The method of claim 1 , wherein the polynucleotide has a poly-A tail of 80-250 nucleotides in length.
6. The method of claim 1 , wherein a second dose of the nucleic acid vaccine is administered to the subject.
7. The method of claim 1 , wherein the mRNA polynucleotide includes a chemical modification selected from the group consisting of pseudouridine, N1-methylpseudouridine, 2-thiouridine, 4′-thiouridine, 5-methylcytosine, 2-thio-1-methyl-1-deaza-pseudouridine, 2-thio-1-methyl-pseudouridine, 2-thio-5-aza-uridine, 2-thio-dihydropseudouridine, 2-thio-dihydrouridine, 2-thio-pseudouridine, 4-methoxy-2-thio-pseudouridine, 4-methoxy-pseudouridine, 4-thio-1-methyl-pseudouridine, 4-thio-pseudouridine, 5-aza-uridine, dihydropseudouridine, 5-methoxyuridine, and 2′-O-methyl uridine.
8. The method of claim 1 , wherein the mRNA polynucleotide includes from about 500 to about 2,000 nucleotides.
9. The method of claim 1 , wherein a single dosage of between 25 ug/kg and 400 ug/kg of the nucleic acid vaccine is administered to the subject in an effective amount to vaccinate the subject.
10. The method of claim 1 , wherein the vaccine is administered to the subject via a route selected from the group consisting of intramuscular administration, intradermal administration and subcutaneous administration.
11. The method of claim 1 , wherein the method of producing an antigen specific immune response involves a single administration of the vaccine.