Non-naturally occurring porcine reproductive and respiratory syndrome virus (PRRSV) and methods of using
A non-naturally occurring porcine reproductive and respiratory syndrome virus (PRRSV) is provided herein, and methods of making and using the non-naturally occurring PRRSV also are provided.
1. A non-structural PRRSV-CON polypeptide having a sequence selected from the group consisting of SEQ ID NO:3, 5, and 7.
2. The polypeptide of claim 1 , wherein the polypeptide is encoded by a nucleic acid, respectively, having a sequence selected from the group consisting of SEQ ID NO:2, 4, and 6.
3. A virus particle comprising the non-structural PPRSV-CON polypeptide of claim 1 .
4. A composition comprising the virus particle of claim 3 and a pharmaceutically acceptable carrier.
5. The composition of claim 4 , further comprising an adjuvant.
6. A composition comprising the non-structural polypeptide of claim 1 and a pharmaceutically acceptable carrier.
7. The composition of claim 6 , further comprising an adjuvant.
8. A method for eliciting an immune response to PPRSV in a porcine, comprising administering, to a porcine:
(i) an effective amount of the polypeptide of claim 1 ;
(ii) an effective amount of the virus particle of claim 3 ; or
(iii) an effective amount of the composition of claim 6 .
9. The method of claim 8 , wherein the administration is selected from the group consisting of intramuscularly, intraperitoneally, and orally.
10. A method for treating or preventing PPRS in a porcine, comprising administering, to a porcine:
(i) an effective amount of the polypeptide of claim 1 ;
(ii) an effective amount of the virus particle of claim 3 ; or
(iii) an effective amount of the composition of claim 6 .
11. The method of claim 10 , wherein the administration is selected from the group consisting of intramuscularly, intraperitoneally, and orally.