System and apparatus for reactions
This disclosure provides systems, apparatuses, and methods for liquid transfer and performing reactions. In one aspect, a system includes a liquid transfer device having a housing having a pipette tip and a plunger assembly; and a reaction chamber, wherein the housing of the liquid transfer device is configured to sealably engage with the reaction chamber. In another aspect, a liquid transfer device including a housing having a pipette tip; and a plunger assembly disposed within the housing and the pipette tip, wherein a portion of the plunger assembly is configured to engage a fluid reservoir such that the plunger assembly remains stationary relative to the fluid reservoir and the housing moves relative to the plunger assembly.
1. A method comprising:
(i) obtaining a liquid sample from a sample reservoir using a liquid transfer device comprising:
a housing comprising a pipette tip; and
a plunger unit disposed within the housing, wherein a portion of the plunger unit is configured to engage a fluid reservoir such that the plunger unit remains stationary relative to the fluid reservoir and the housing moves relative to the plunger unit to draw a fluid from the fluid reservoir through the pipette tip; and
(ii) dispensing the liquid sample.
2. The method of claim 1 , wherein dispensing the liquid sample comprises dispensing the liquid sample into a reaction chamber comprising one or more components of a reaction.
3. The method of claim 1 , the plunger unit including a syringe plunger that seals within the pipette tip with an o-ring.
4. The method of claim 1 , wherein movement of the housing relative to the plunger unit results in creation of a vacuum within the pipette tip.
5. The method of claim 1 , wherein the housing is configured to move relative to the plunger unit when the housing is advanced toward the fluid reservoir.
6. The method of claim 4 , wherein the plunger unit is configured to lock in a position resulting in creation of the vacuum.
7. The method of claim 4 , wherein the device is configured to provide at least one of an auditory and visual indication that the plunger unit is in a position resulting in the creation of the vacuum.
8. The method of claim 1 , wherein the plunger unit is configured to reversibly lock in a position that causes fluid from the fluid reservoir to flow into the pipette tip.