Instrument for cassette for sample preparation
A parallel processing system for processing samples is described. In one embodiment, the parallel processing system includes an instrument interface parallel controller to control a tray motor driving system, a close-loop heater control and direction system, a magnetic particle transfer system, a reagent release system, a reagent pre-mix pumping system and a wash buffer pumping system.
1. A method, comprising:
(a) disposing a cassette in an instrument enclosure, the cassette comprising:
(i) a first chamber;
(ii) a second chamber;
(iii) a valve comprising a stationary piece and a movable piece, the movable piece defining a cavity within which magnetic particles can be disposed, the valve configured to transfer the magnetic particles between the first chamber and the second chamber while maintaining fluid isolation between the first chamber and the second chamber;
(iv) a third chamber; and
(v) a fourth chamber;
(b) actuating a first plunger disposed within the third chamber of the cassette to transfer a first reagent from the third chamber into the first chamber, wherein the first plunger comprises a first sealing element to prevent leakage of first reagent until the first plunger is moved;
(c) actuating a second plunger disposed within the fourth chamber of the cassette to transfer a second reagent from the fourth chamber into the first chamber, wherein the second plunger comprises a second sealing element to prevent leakage of second reagent until the second plunger is moved;
(d) applying a magnetic force to the valve to attract the magnetic particles into the cavity of the movable piece of the valve; and
(e) moving the movable piece of the valve relative to the stationary piece of the valve such that the magnetic particles are transferred between the first chamber and the second chamber while maintaining fluid isolation between the first chamber and the second chamber.
2. The method of claim 1 , further comprising washing the magnetic particles in a washing solution in the second chamber of the cassette.
3. The method of claim 1 , further comprising heating the first chamber.
4. The method of claim 1 , wherein the cassette further comprises a fifth chamber comprising a sealing element defining at least a portion of a boundary between the fifth chamber and the first chamber, the method further comprising actuating a third plunger associated with the fifth chamber of the cassette to transfer a third reagent from the fifth chamber into the first chamber.
5. The method of claim 1 , wherein the cassette further comprises a pump configured to mix at least the first reagent and the second reagent in the first chamber, the method further comprising actuating the pump.
6. A method, comprising:
(a) disposing a cassette in an instrument enclosure, the cassette comprising:
(i) a sample chamber containing a sample;
(ii) a wash chamber;
(iii) a valve comprising a stationary piece and a movable piece, the movable piece defining a cavity within which magnetic particles can be disposed, the valve configured to transfer the magnetic particles between the sample chamber and the wash chamber while maintaining fluid isolation between the sample chamber and the wash chamber; and
(iv) a first reagent chamber; and
(v) a magnetic particle chamber;
(b) actuating a first plunger disposed within the first reagent chamber of the cassette to transfer a first reagent from the first reagent chamber into the sample chamber, wherein the first plunger comprises a first sealing element configured to prevent leakage of the first reagent until the first plunger is moved;
(c) actuating a second plunger disposed within the magnetic particle chamber of the cassette to transfer magnetic particles from the magnetic particle chamber into the sample chamber, wherein the second plunger comprises a second sealing element configured to prevent leakage of the magnetic particles until the second plunger is moved;
(d) applying a magnetic force to the valve to attract the magnetic particles into the cavity of the movable piece of the valve; and
(e) moving the movable piece of the valve relative to the stationary piece of the valve such that the magnetic particles are transferred between the sample chamber and the wash chamber while maintaining fluid isolation between the sample chamber and the wash chamber.
7. The method of claim 6 , wherein the sample is a blood or urine sample.
8. The method of claim 6 , wherein the sample comprises cells.
9. The method of claim 8 , wherein the cells are human cells, bacteria cells, or yeast cells.
10. The method of claim 6 , wherein the first reagent is a lysis solution.
11. The method of claim 6 , wherein the cassette further comprises at least a second reagent chamber comprising a sealing element defining at least a portion of a boundary between the second reagent chamber and the sample chamber, the method further comprising actuating a third plunger associated with the second reagent chamber of the cassette to transfer the second reagent from the second reagent chamber into the sample chamber.
12. The method of claim 11 , wherein the second reagent comprises proteinase K or a binding solution.
13. The method of claim 6 , further comprising heating the sample and one or more of a lysis solution, proteinase K, and magnetic particles in the sample chamber.
14. The method of claim 6 , wherein the cassette further comprises a pump configured to mix the sample and at least the first reagent in the sample chamber, the method further comprising actuating the pump.
15. The method of claim 6 , wherein the valve is a first valve, the wash chamber is a first wash chamber, and the cassette further comprises a second wash chamber, and a second valve comprising a stationary piece and a movable piece defining a cavity within which the magnetic particles can be disposed, the second valve configured to transfer the magnetic particles between the first wash chamber and the second wash chamber while maintaining fluid isolation between the first wash chamber and the second wash chamber, the method further comprising applying a magnetic force to the second valve to attract the magnetic particles into the cavity of the movable piece of the second valve; and moving the movable piece of the second valve relative to the stationary piece of the second valve such that the magnetic particles are transferred between the first wash chamber and the second wash chamber while maintaining fluid isolation between the first wash chamber and the second wash chamber.
16. The method of claim 15 , wherein the cassette further comprises an elution chamber and a third valve comprising a stationary piece and a movable piece defining a cavity within which the magnetic particles can be disposed, the method comprising applying a magnetic force to the third valve to attract the magnetic particles into the cavity of the movable piece of the third valve; and moving the movable piece of the third valve relative to the stationary piece of the valve such that the magnetic particles are transferred between the second wash chamber and the elution chamber.
17. The method of claim 16 , further comprising mixing the magnetic particles and an elution buffer in the elution chamber.
18. The method of claim 17 , further comprising heating the magnetic particles and the elution buffer in the elution chamber.