In vitro growth of tissues suitable to the formation of bone and bone forming tissue formed thereby
The present invention is directed to a device for the growth of new bone or bone-like tissue under in vitro cell culture conditions.
1. An allogenic bone material composition for implantation in a human patient, comprising:
(a) human demineralized cortical bone,
(b) human non-demineralized bone, and
(c) osteocytes allogenic to the human patient,
wherein the allogenic bone material composition is frozen and stored in a container.
2. The allogenic bone material composition of claim 1 , further comprising osteoblasts.
3. The allogenic bone material composition of claim 1 , wherein the composition further comprises stem cells.
4. The allogenic bone material composition of claim 1 , wherein the osteocytes are suitable for forming an osteoid.
5. The allogenic bone material composition of claim 1 , wherein the demineralized cortical bone is in the form of particles.
6. The allogenic bone material composition of claim 5 , wherein the particles have a size of 0.05-4 mm.
7. The allogenic bone material composition of claim 5 , wherein the particles have a size of 0.25-0.71 mm.
8. The allogenic bone material composition of claim 5 , wherein the particles have a size of 0.125-0.85 mm.
9. The allogenic bone material composition of claim 1 , wherein the demineralized cortical bone is in the form of fibers.
10. The allogenic bone material composition of claim 9 , wherein the fibers have a length of 1-500 mm.
11. The allogenic bone material composition of claim 1 , wherein the demineralized cortical bone contains calcium at a level of from 1 wt % to 4 wt %.
12. The allogenic bone material composition of claim 1 , wherein the non-demineralized bone is in the form of particles.
13. The allogenic bone material composition of claim 1 , wherein the non-demineralized bone is in the form of fibers.
14. The allogenic bone material composition of claim 1 , further comprising a growth factor.
15. The allogenic bone material composition of claim 1 , further comprising a differentiation factor.
16. The allogenic bone material composition of claim 1 , further comprising an agent wherein the agent is selected from the group consisting of Dulbecco's modified Eagle's medium, antibiotics, dexamethasone, beta-glycerolphosphate, glucose, glutamine, amino acid supplements, antioxidants, L-ascorbic acid, culture medium, serum and a combination thereof.
17. The allogenic bone material composition of claim 1 , further comprising antibiotics, glucose, amino acid supplements, or a combination thereof.