Relaxin immunoglobulin fusion proteins and methods of use
Disclosed herein are relaxin immunoglobulin fusion proteins useful for the treatment or prevention of a disease or condition in a subject.
1. An immunoglobulin fusion protein comprising a therapeutic peptide inserted into a light chain complementarity determining region 3 (CDR3) of an antibody variable region comprising: a heavy chain comprising a CDR1 of SEQ ID NO: 72, a CDR2 of SEQ ID NO: 73, and a CDR3 sequence selected from SEQ ID NOS: 75-78; and a light chain comprising a CDR1 of SEQ ID NO: 89, a CDR2 of SEQ ID NO: 90, and a CDR3 sequence comprising at least three contiguous amino acids of SEQ ID NO: 91.
2. The immunoglobulin fusion protein of claim 1 , comprising SEQ ID NO: 75: SMITX(1)X(2)X(3)FDV; wherein X(1) is F, A, G, or P; X(2) is G, A, S, T, or P; and X(3) is G, A, V, L, or P.
3. The immunoglobulin fusion protein of claim 2 , wherein X(1) is F.
4. The immunoglobulin fusion protein of claim 2 , wherein X(2) is G.
5. The immunoglobulin fusion protein of claim 2 , wherein X(3) is G.
6. The immunoglobulin fusion protein of claim 1 , wherein the sequence selected from SEQ ID NOS: 75-78 comprises a sequence selected from SEQ ID NOS: 79-88.
7. The immunoglobulin fusion protein of claim 6 , comprising the SEQ ID NO: 79.
8. A method comprising administering to a subject in need thereof the immunoglobulin fusion protein of claim 1 .
9. An antibody comprising the immunoglobulin fusion protein of claim 1 and a Fc domain at least 90% homologous to SEQ ID NO: 98.
10. The antibody of claim 9 , comprising SEQ ID NO: 16.
11. An antibody comprising a Fc region having SEQ ID NO: 98, and an antibody variable region comprising a heavy chain comprising a sequence at least about 95% identical to SEQ ID NO: 16, and a light chain comprising a therapeutic peptide and a sequence at least about 95% identical to SEQ ID NO: 9.
12. The antibody of claim 11 , wherein the therapeutic peptide is positioned within the antibody variable region.
13. The antibody of claim 11 , wherein the therapeutic peptide is inserted into the antibody variable region.