Decellularized placental membrane and methods of preparing and use thereof
A method of preparing a decellularized placental membrane is provided. The method comprises removing cells from a pre-decellularized placental membrane comprising an amnion layer and a chorion layer to produce a decellularized placental membrane without separating the amnion layer from the chorion layer. The pre-decellularized placental membrane is obtained from an amniotic sac, and the decellularized placental membrane comprises the amnion layer and the chorion layer. Also provided is a decellularized placental membrane and a placenta-derived graft comprising the decellularized placental membrane. Further provided are the uses of the decellularized placental membrane or the placenta-derived graft.
1. A method of preparing a decellularized placental membrane, comprising
(a) obtaining an amniotic sac cut from a placenta around a placenta skirt, wherein the amniotic sac comprises a pre-decellularized placental membrane and the pre-decellularized placental membrane comprises an amnion layer and a chorion layer,
(b) treating the pre-decellularized placental membrane with one or more compounds selected from the group consisting of N-lauroylsarcosinate, n-octyl-b-D-glucopyranoside, a polyoxyethylene alcohol, a polyoxyethylene isoalcohol, a polyoxyethylene p-t-octyl phenol, a polyoxyethylene nonyphenol, a polyoxyethylene ester of a fatty acid, and a polyoxyethylene sorbitol ester, and
(c) removing cells from the pre-decellularized placental membrane without separating the amnion layer from the chorion layer, wherein the cells comprise cells in the amnion layer and the chorion layer in the pre-decellularized placental membrane, whereby a decellularized placental membrane is prepared.
2. The method of claim 1 , wherein the decellularized placental membrane comprises one or more growth factors in an amount that is at least 10% greater than the sum of the amount of the one or more growth factors in a decellularized control isolated amniotic membrane having the same size as the decellularized placental membrane and the amount of the one or more growth factors in a decellularized control isolated chorionic membrane having the same size as the decellularized placental membrane, wherein the decellularized control isolated amniotic membrane is prepared by removing cells from the amnion layer isolated from the pre-decellularized placental membrane in the same manner as the decellularized placental membrane is prepared from the pre-decellularized placental membrane, and wherein the decellularized control isolated chorion is prepared by removing cells from the chorion layer isolated from the pre-decellularized placental membrane in the same manner as the decellularized placental membrane is prepared from the pre-decellularized placental membrane.
3. The method of claim 1 , wherein the decellularized placental membrane comprises one or more growth factors in an amount that is at least 20% of the amount of the one or more growth factors in the pre-decellularized placental membrane.
4. The method of claim 1 , wherein the decellularized placental membrane comprises less than 100 ng dsDNA per mg dry weight of the decellularized placental membrane.
5. The method of claim 1 , wherein the decellularized placental membrane comprises DNA in an amount that is less than 10% of the DNA in the pre-decellularized placental membrane.
6. The method of claim 1 , wherein the amnion layer in the pre-decellularized placental membrane comprises a fibroblast layer, and wherein the chorion layer in the pre-decellularized placental membrane comprises a reticular layer.
7. The method of claim 1 , wherein the amnion layer in the pre-decellularized placental membrane comprises epithelium, a basement membrane, a compact layer, a fibroblast layer, and a spongy layer, and wherein the chorion layer in the pre-decellularized placental membrane comprises a cellular layer, a reticular layer, a pseudo-basement membrane, and a trophoblast layer.
8. The method of claim 1 , further comprising harvesting the amniotic sac from a donor.
9. The method of claim 1 , further comprising cleaning and disinfecting the pre-decellularized placental membrane.
10. The method of claim 1 , further comprising fixing the pre-decellularized placental membrane onto a frame before the cell removal step.
11. The method of claim 1 , wherein the chorion layer in the pre-decellularized placental membrane comprises a trophoblast layer.
12. The method of claim 1 , wherein the one or more compounds are present in the composition at a concentration of 0.01-5% (w/v).
13. The method of claim 1 , further comprising treating the pre-decellularized placental membrane with one or more endonucleases at a concentration of 20-400 U/mL.
14. A decellularized placental membrane prepared by the method of claim 1 .
15. A placenta-derived graft comprising the decellularized placental membrane of claim 14 and one or more agents.
16. A method comprising culturing cells in the presence of the decellularized placental membrane prepared by the method of claim 1 .
17. A method of promoting differentiation of pluripotent stem cells or tissue-specific progenitor cells, comprising culturing the pluripotent stem cells or tissue-specific progenitor cells in the presence of an effective amount of the decellularized placental membrane prepared by the method of claim 1 .
18. A method of repairing a defect in a tissue, comprising contacting the site of the defect with an effective amount of the decellularized placental membrane prepared by the method of claim 1 .