Cell harvesting apparatus
Disclosed herein is a cell harvesting instrument suitable for concentrating cells from a source suspension of cells and/or washing said cells, the instrument comprising: a housing for accommodating mechanical elements including at least one fluid pump, at least one valve; and a processing kit removably insertable into the housing, said kit including a generally flat frame having or supporting plural sealed fluid paths arranged in a generally flat plane and such that fluids in the paths do not contact said mechanical elements, wherein at least portions of the fluid paths comprise flexible tubes, the outer surfaces of which are manipulateable by the or each fluid pump, to provide fluid flow in one or more of the paths and/or by the or each valve to restrict fluid flow in one or more of the paths. In an embodiment, the kit comprises also a fluid processing reservoir and a filter suitable for separating cells from fluid in said paths. A transfer mechanism for moving and weighing the fluid processing reservoir is disclosed also.
1. A cell harvesting processing kit for at least one of separating cells from fluids and washing cells, the kit comprising:
sealed fluid paths, at least a portion of which are formed from flexible tubing suitable for being externally manipulated by mechanical elements,
a fluid processing reservoir and a filter suitable for retaining cells whilst permeating fluids in said paths,
wherein the fluid paths are supported by a generally flat supporting frame such that said paths, reservoir and filter are arranged in a generally flat plane; and
wherein said supporting frame includes through-apertures, and said flexible tubing is arranged to extend across said apertures such that the tubing is accessible from both sides of the flat supporting frame to allow said external manipulation from both sides of the frame.
2. The kit of claim 1 , wherein said fluid processing reservoir is in the form of a container which lies generally flat in the frame and is demountable from the frame and re-attachable to the frame.
3. The kit of claim 1 , wherein said fluid paths include at least one inlet located at the ends of extended flexible tubing for further fluid connection remote from the frame.
4. The kit of claim 3 , wherein the plural fluid paths and extended fluid paths form a closed fluid processing system arranged such that fluids in the paths do not contact mechanical elements which effect fluid control.
5. The kit of claim 3 , wherein said fluid paths further include at least one outlet.
6. The kit of claim 3 , wherein the plural fluid paths and extended fluid paths form a closed fluid processing system arranged such that fluids in the paths do not contact at least one of a fluid pump or pumps and a fluid valve or valves.