Cell harvesting apparatus
Disclosed herein is a cell harvesting instrument suitable for concentrating cells from a source suspension of cells and/or washing said cells, the instrument comprising: a housing for accommodating mechanical elements including at least one fluid pump, at least one valve; and a processing kit removably insertable into the housing, said kit including a generally flat frame having or supporting plural sealed fluid paths arranged in a generally flat plane and such that fluids in the paths do not contact said mechanical elements, wherein at least portions of the fluid paths comprise flexible tubes, the outer surfaces of which are manipulateable by the or each fluid pump, to provide fluid flow in one or more of the paths and/or by the or each valve to restrict fluid flow in one or more of the paths. In an embodiment, the kit comprises also a fluid processing reservoir and a filter suitable for separating cells from fluid in said paths. A transfer mechanism for moving and weighing the fluid processing reservoir is disclosed also.
1. A pinch valve mechanism operable to pinch a flexible tube against a reaction surface, the mechanism comprising:
at least one pivotable arm including on one side a finger to perform said pinching and on an opposing side a cam profile, and
a linearly moveable actuator in use acting on the or each cam profile to move an associated finger,
wherein the at least one pivotable arm comprises a first arm and a second arm each operable to pinch a different tube,
wherein the first arm and the second arm pivot about a same pivot axis,
wherein the cam profiles of the first and second arms are the same or similar in profile but opposed in orientation such that when a first finger of the first arm provides a pinching action a second finger of the second arm provides no pinching action, and vice versa.
2. The mechanism of claim 1 , wherein the first and second arms share the linearly moveable actuator.
3. The mechanism of claim 1 , wherein the opposed cam profiles of the first and second arms have a common mid- position at which both fingers provide a pinching action.
4. A pinch valve including the pinch valve mechanism of claim 1 , and the reaction surface arranged to oppose a force of said pinching.
5. The pinch valve of claim 4 , wherein the pinch valve is mounted to a pivotable assembly for movement into, and out of, an operative position.
6. A cell harvesting instrument comprising a housing and a processing kit removably mounted therein configured for concentrating and/or washing the cells and comprising a filter and the flexible tube defining a portion of a fluid path therethrough and the pinch valve mechanism of claim 1 .
7. A pinch valve including the pinch valve mechanism of claim 2 , and the reaction surface arranged to oppose a force of said pinching.
8. A method for at least one of concentrating and washing cells the method comprising, using the cell harvesting instrument of claim 6 for conducting at least one of concentrating and washing cells.
9. The method of claim 8 , wherein the cells are human cells.