Cell harvesting apparatus
Disclosed herein is a cell harvesting instrument suitable for concentrating cells from a source suspension of cells and/or washing said cells, the instrument comprising: a housing for accommodating mechanical elements including at least one fluid pump, at least one valve; and a processing kit removably insertable into the housing, said kit including a generally flat frame having or supporting plural sealed fluid paths arranged in a generally flat plane and such that fluids in the paths do not contact said mechanical elements, wherein at least portions of the fluid paths comprise flexible tubes, the outer surfaces of which are manipulateable by the or each fluid pump, to provide fluid flow in one or more of the paths and/or by the or each valve to restrict fluid flow in one or more of the paths. In an embodiment, the kit comprises also a fluid processing reservoir and a filter suitable for separating cells from fluid in said paths. A transfer mechanism for moving and weighing the fluid processing reservoir is disclosed also.
1. A transfer mechanism for transferring a fluid processing reservoir from a support so that the volume of liquids in the reservoir can be estimated in use, the mechanism comprising:
a weighing member comprising a hook and a load cell, wherein the hook is operationally supported by and positioned below the load cell; and
a front carriage comprising an extension moveable by an actuator, the extension pushing the front carriage in a forward direction toward the reservoir and pulling the front carriage in a rearward direction away from the reservoir, the front carriage collecting the entire reservoir in the forward direction and hanging the entire reservoir on the hook of the weighing member as a result of moving in the rearward direction;
wherein the weighing member is configured to allow the reservoir to hang freely below the hook, such that the weighing member is effective to measure an in-use weight of the reservoir via a force applied to the load cell when the reservoir is hanging freely below the hook.
2. The mechanism of claim 1 , wherein a single actuator is employed for said forward and rearward movements and said collecting and said hanging operations.
3. The mechanism of claim 2 , wherein said front carriage is driven by a rear carriage, wherein the rear carriage is driven by the actuator.
4. The mechanism of claim 3 , wherein in the forward pushing direction, the extension acting on a sprung latch mounted to the front carriage having a spring force which is not overcome by said pushing, in a collecting operation the extension overcoming the spring force of the latch to engage the latch with the reservoir for said collecting operation, and in the hanging operation the extension acting to pull the front carriage rearwards and acting to withdrawing the latch from the reservoir to hang the reservoir on the hook with no contact between the reservoir and the latch.
5. The mechanism of claim 4 , wherein the mechanism provides a return operation for returning the reservoir from the hook to the support, the return operation including returning the latch of the front carriage into engagement with the reservoir, pushing the extension, together with the front carriage and reservoir forwards, and moving the extension beyond the collecting position to unlatch the sprung latch from the reservoir, and to reengage the reservoir and its support.
6. The mechanism of claim 1 , wherein said front carriage is driven by a rear carriage, in turn driven by the actuator having a lead screw.
7. A cell harvesting instrument, including a transfer mechanism of claim 1 .