Peptide tag and tagged protein including same
A peptide comprising the sequence shown below is added as a peptide tag to a useful protein, followed by allowing its expression. X m (PY n ) q PZ r In this formula, X, Y, and Z each represent an amino acid residue independently selected from the group consisting of R, G, S, K, T, L, N, Q, and H, with the proviso that at least one Y represents K, L, N, Q, H, or R. m represents an integer of 0 to 5; n represents 1, 2, or 3; q represents an integer of 1 to 10; and r represents an integer of 0 to 10.
1. A peptide comprising the amino acid sequence of SEQ ID NO: 25, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 54, 64, 66, 102, 104, 106, 112, 114, 116, 118, 122, 124, 132, 136, 140, 142, 147, 149, 151, 153, or 157.
2. A tagged protein comprising:
the peptide according to claim 1 and
a protein.
3. The tagged protein according to claim 2 ,
wherein the protein is selected from the group consisting of human growth hormone, interferon β, xylanase, esterase, and green fluorescent protein (GFP).
4. The tagged protein according to claim 2 , wherein the peptide is linked to the protein through a protease recognition sequence.
5. The tagged protein according to claim 2 , further comprising:
a secretion signal.
6. A DNA encoding the tagged protein according to claim 2 .
7. A recombinant vector comprising:
the DNA according to claim 6 .
8. A transformant prepared by transformation with the DNA according to claim 6 .
9. The transformant according to claim 8 ,
wherein the transformant is yeast, E. coli, Brevibacillus , an insect cell, or a mammalian cell, and
wherein the mammalian cell comprises a human cultured cell, but does not comprise a human individual.
10. A method for producing a tagged protein, comprising:
culturing the transformant according to claim 8 to allow accumulation of the tagged protein, and
collecting the tagged protein.
11. A transformant prepared by transformation with the recombinant vector according to claim 7 .
12. A peptide consisting of the amino acid sequence of SEQ ID NO: 52, 58, 60, 92, 94, 96, 100, 108, 110, 120, 128, 130, 134, 145, or 155.
13. The peptide of claim 12 , wherein peptide consists of the amino acid sequence of SEQ ID NO: 52, 58, 60, 92, 94, 96, 100, 108, 110, 120, 130, 134, 145, or 155.