IP Library Granted Patent US 10,822,311
Granted Patent B2
US 10,822,311 · App. 16/077,904 · Granted Nov 3, 2020

Pyrimidines and variants thereof, and uses therefor

Inventors: Ronald Charles Hawley (Oakland, CA); Prabha Ibrahim (Mountain View, CA); Anthony P. Ford (Palo Alto, CA); Joel R. Gever (Los Altos, CA)
Assignee: Afferent Pharmaceuticals, Inc.
C07D239/48C07D401/12C07D403/12C07D405/12
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Quick Facts
Patent No.
US 10,822,311
App. No.
16/077,904
Granted
Nov 3, 2020
Kind
B2
Abstract

The present disclosure provides pyrimidine compounds and uses thereof, for example, for the treatment of diseases associated with P2X purinergic receptors. In certain aspects, the present disclosure provides P2X3 and/or P2X2/3 antagonists which are useful, for example, for the treatment of visceral organ, cardiovascular and pain-related diseases, conditions and disorders.

Claims (78)

1. A compound of Formula 1:

or a pharmaceutically acceptable salt thereof, wherein:

W is O or S;

X 1 is N or CR 2 ;

X 2 is CR 3 ;

X 3 is CR 4 ;

X 4 is CR 5 ;

X 5 is N or CR 6 , provided, however, when X 1 is CR 2 , W is not O;

Y is selected from hydrogen or —NR d R e , wherein one of R d and R e is hydrogen, and the other is: hydrogen; C 1-12 -alkyl; C 3-12 -cycloalkyl; C 4-12 -cycloalkylalkyl; C 1-12 -haloalkyl; C 1-12 -haloalkoxy; C 1-12 -hydroxyalkyl; C 2-12 -alkoxyalkyl; acetyl; C 1-12 -alkylsulfonyl; C 2-12 -alkylsulfonylalkyl; C 2-12 -aminocarbonyloxyalkyl; C 1-12 -hydroxycarbonylalkyl; C 2-12 -hydroxylalkyloxycarbonylalkyl; C 5-12 -aryl; C 6-12 -arylalkyl; C 5-12 -arylsulfonyl; C 5-12 -heteroaryl; C 6-12 -heteroarylalkyl; C 5-12 -heteroarylsulfonyl; C 3-12 -heterocyclyl; or C 4-12 -heterocyclylalkyl;

D is an optional oxygen;

R 1 is C 1-12 -alkyl;

R 2 and R 4 are each independently selected from hydrogen; C 1-12 -alkyl; C 2-12 -alkenyl; C 2-12 -alkynyl; amino; halo; amido; C 1-12 -haloalkyl; C 1-12 -alkoxy; hydroxy; C 1-12 -haloalkoxy; nitro; C 1-12 -hydroxyalkyl; C 2-12 -alkoxyalkyl; C 1-12 -hydroxyalkoxy; C 3-12 -alkynylalkoxy; C 1-12- alkylsulfonyl; C 5-12 -arylsulfonyl; cyano; C 6-12 -aryl; C 5-12 -heteroaryl; C 3-12 -heterocyclyl; C 4-12 -heterocyclylalkoxy; C 6-12 -aryloxy; C 5-12 -heteroaryloxy; C 7-12 -arylalkyloxy; C 6-12 -heteroaralkyloxy; optionally substituted phenoxy; —(CH 2 )m-(Z)n-(CO)—R f and —(CH 2 )m-(Z)n-SO 2 —(NR g )n′-R f , where m, n and n′ are each independently 0 or 1;

R 3 is C 1-12 -alkoxy;

R 5 is hydrogen;

Z is O or NR g ;

R f is selected from hydrogen, C 1-12 -alkyl, hydroxy, C 1-12 -alkoxy, amino, C 1-12 -hydroxyalkyl and C 2-12 -alkoxyalkyl;

each R g is independently hydrogen or C 1-12 -alkyl;

or alternatively, R 3 and R 4 together with the atoms to which they are attached form a five or six-membered ring that optionally includes one or two heteroatoms independently selected from O, S and N;

or alternatively, R 2 and R 3 together form an alkylene dioxy; or R 2 and R 3 together with the atoms to which they are attached may form a five or six-membered ring that optionally includes one or two heteroatoms independently selected from O, S and N;

R 6 is selected from hydrogen and C 1-12 -alkyl; and

R 7 is selected from hydrogen; C 1-12 -alkyl; C 3-12 -cycloalkyl; C 4-12 -cycloalkylalkyl; C 1-12 -haloalkyl; C 1-12 -haloalkoxy; C 1-12 -hydroxyalkyl; C 2-12 -alkoxyalkyl; acetyl; C 1-12 -alkylsulfonyl; C 2-12 -alkylsulfonylalkyl; C 2-12 -aminocarbonyloxyalkyl; C 2-12 -hydroxycarbonylalkyl; C 3-12 -hydroxyalkyloxycarbonylalkyl; C 6-12 -aryl; C 7-12 -arylalkyl; C 6-12 -arylsulfonyl; C 5-12 -heteroaryl; C 6-12 -heteroarylalkyl; C 5-12 -heteroarylsulfonyl; C 3-12 -heterocyclyl; and C 4-12 -heterocyclylalkyl.

2. The compound of claim 1 , or a pharmaceutically acceptable salt thereof, wherein X 1 is CR 2 and W is S, providing compounds of Formula 1a as follows:

3. The compound of claim 1 , or a pharmaceutically acceptable salt thereof, wherein X 1 is N, providing compounds of the Formula 1b, as follows:

4. The compound of claim 1 , or a pharmaceutically acceptable salt thereof, wherein X 5 is CR 6 , providing compounds of Formula 1m, as follows:

provided, however, when X 1 is CR 2 , W is not O.

5. The compound of claim 1 , or a pharmaceutically acceptable salt thereof, wherein X 2 and X 3 of Formula I are each C—OMe, providing compounds of Formula 1n, as follows:

6. The compound of claim 1 , or a pharmaceutically acceptable salt thereof, wherein W is O.

7. The compound of claim 1 , or a pharmaceutically acceptable salt thereof, wherein W is S.

8. The compound of claim 1 of Formula 2, or a pharmaceutically acceptable salt thereof:

wherein:

R 1 is C 1-12 -alkyl;

R 3 is C 1-12 -alkoxy;

R 4 is: hydrogen; C 1-12 -alkyl; C 2-12 -alkenyl; C 2-12 -alkynyl; amino; halo; amido; C 1-12 -haloalkyl; C 1-12 -alkoxy; hydroxy; C 1-12 -haloalkoxy; nitro; C 1-12 -hydroxyalkyl; C 2-12 -alkoxyalkyl; C 1-12 -hydroxyalkoxy; C 3-12 -alkynylalkoxy; C 2-12 -alkylsulfonyl; C 6-12 -arylsulfonyl; cyano; C 6-12 -aryl; C 5-12 -heteroaryl; C 3-12 -heterocyclyl; C 4-12 -heterocyclylalkoxy; C 6-12 -aryloxy; C 5-12 -heteroaryloxy; C 7-12 -arylalkyloxy; C 6-12 -heteroarylalkyloxy; optionally substituted phenoxy; —(CH 2 )m-(Z)n-(CO)—R f or —(CH 2 )m-(Z)n-SO 2 —(NR g )n′-R f , where m, n and n′ are each independently 0 or 1;

Z is O or NR g ;

R f is hydrogen, C 1-12 -alkyl, hydroxy, C 1-12 -alkoxy, amino, C 1-12 -hydroxyalkyl or C 2-12 -alkoxyalkyl;

each R g is independently hydrogen or C 1-12 -alkyl;

or alternatively, R 3 and R 4 together with the atoms to which they are attached may form a five or six-membered ring that optionally includes one or two heteroatoms independently selected from O, S and N;

R 7 is selected from hydrogen; C 1-12 -alkyl; C 3-12 -cycloalkyl; C 4-12 -cycloalkylalkyl; C 1-12 -haloalkyl; C 1-12 -haloalkoxy; C 1-12 -hydroxyalkyl; C 2-12 -alkoxyalkyl; acetyl; C 1-12 -alkylsulfonyl; C 2-12 -alkylsulfonylalkyl; C 2-12 -aminocarbonyloxyalkyl; C 2-12 -hydroxycarbonylalkyl; C 2-12 -hydroxyalkyloxycarbonylalkyl; C 6-12 -aryl; C 7-12 -arylalkyl; C 6-12 -arylsulfonyl; C 5-12 -heteroaryl; C 6-12 -heteroarylalkyl; C 5-12 -heteroarylsulfonyl; C 3-12 -heterocyclyl; and C 4-12 -heterocyclylalkyl; and

R d is selected from hydrogen; C 1-12 -alkyl; C 3-12 -cycloalkyl; C 4-12 -cycloalkylalkyl; C 1-12 -haloalkyl; C 1-12 -haloalkoxy; C 1-12 -hydroxyalky; C 2-12 -alkoxyalkyl; acetyl; C 1-12 -alkylsulfonyl; C 2-12 -alkylsulfonylalkyl; C 2-12 -aminocarbonyloxyalkyl; C 2-12 -hydroxycarbonylalkyl; C 2-12 -hydroxyalkyloxycarbonylalkyl; C 6-12 -aryl; C 7-12 -arylalkyl; C 6-12 -arylsulfonyl; C 5-12 -heteroaryl; C 6-12 -heteroarylalkyl; C 5-12 -heteroarylsulfonyl; C 3-12 -heterocyclyl; and C 4-12 -heterocyclylalkyl.

9. A compound of Formula 3, or a pharmaceutically acceptable salt thereof:

wherein:

R 3 is C 1-12 -alkoxy;

R 4 is: hydrogen; C 1-12 -alkyl; C 2-12 -alkenyl; C 2-12 -alkynyl; amino; halo; amido; C 1-12 -haloalkyl; C 1-12 -alkoxy; hydroxy; C 1-12 -haloalkoxy; nitro; C 1-12 -hydroxyalkyl; C 2-12 -alkoxyalkyl; C 1-12 -hydroxyalkoxy; C 3-12 -alkynylalkoxy; C 1-12 -alkylsulfonyl; C 6-12 -arylsulfonyl; cyano; C 6-12 -aryl; C 5-12 -heteroaryl; C 3-12 -heterocyclyl; C 4-12 -heterocyclylalkoxy; C 6-12 -aryloxy; C 5-12 -heteroaryloxy; C 7-12 -arylalkyloxy; C 6-12 -heteroaralkyloxy; optionally substituted phenoxy; —(CH 2 )m-(Z)n-(CO)—R f or —(CH 2 )m-(Z)n-SO 2 —(NR g )n′-R f , where m, n and n′ are each independently 0 or 1;

Z is O or NR g ;

R f is hydrogen, C 1-12 -alkyl, hydroxy, C 1-12 -alkoxy, amino, C 1-12 -hydroxyalkyl or C 2-12 -alkoxyalkyl, and each R g is independently hydrogen or C 1-12 -alkyl;

or alternatively, R 3 and R 4 together with the atoms to which they are attached may form a five or six-membered ring that optionally includes one or two heteroatoms independently selected from O, S and N;

R 7 is selected from: hydrogen; C 1-12 -alkyl; C 3-12 -cycloalkyl; C 4-12 -cycloalkylalkyl; C 1-12 -haloalkyl; C 1-12 -haloalkoxy; C 1-12 -hydroxyalky; C 2-12 -alkoxyalkyl; acetyl; C 1-12 -alkylsulfonyl; C 2-12 -alkylsulfonylalkyl; C 2-12 -aminocarbonyloxyalkyl; C 2-12 -hydroxycarbonylalkyl; C 2-12 -hydroxyalkyloxycarbonylalkyl; C 6-12 -aryl; C 7-12 -arylalkyl; C 6-12 -arylsulfonyl; C 5-12 -heteroaryl; C 6-12 -heteroarylalkyl; C 5-12 -heteroarylsulfonyl; C 3-12 -heterocyclyl; and C 4-12 -heterocyclylalkyl; and

R d is selected from: hydrogen; C 1-12 -alkyl; C 3-12 -cycloalkyl; C 4-12 -cycloalkylalkyl; C 1-12 -haloalkyl; C 1-12 -haloalkoxy; C 1-12 -hydroxyalky; C 2-12 -alkoxyalkyl; acetyl; C 1-12 -alkylsulfonyl; C 2-12 -alkylsulfonylalkyl; C 2-12 -aminocarbonyloxyalkyl; C 2-12 -hydroxycarbonylalkyl; C 2-12 -hydroxyalkyloxycarbonylalkyl; C 6-12 -aryl; C 7-12 -arylalkyl; C 6-12 -arylsulfonyl; C 5-12 -heteroaryl; C 6-12 -heteroarylalkyl; C 5-12 -heteroarylsulfonyl; C 3-12 -heterocyclyl; and C 4-12 -heterocyclylalkyl.

10. The compound of claim 1 of Formula 4, or a pharmaceutically acceptable salt thereof:

wherein:

R 1 is C 1-12 -alkyl;

R 2 is hydrogen; C 1-12 -alkyl; C 2-12 -alkenyl; C 2-12 -alkynyl; amino; halo; amido; C 1-12 -haloalkyl; C 1-12 -alkoxy; hydroxy; C 1-12 -haloalkoxy; nitro; C 1-12 -hydroxyalkyl; C 2-12 -alkoxyalkyl; C 1-12 -hydroxyalkoxy; C 3-12 -alkynylalkoxy; C 1-12 -alkylsulfonyl; C 6-12 -arylsulfonyl; cyano; C 6-12 -aryl; C 5-12 -heteroaryl; C 3-12 -heterocyclyl; C 4-12 -heterocyclylalkoxy; C 6-12 -aryloxy; C 5-12 -heteroaryloxy; C 7-12 -arylalkyloxy; C 6-12 -heteroarylalkyloxy; optionally substituted phenoxy; or —(CH 2 )m-(Z)n-(CO)—R f or —(CH 2 )m-(Z)n-SO 2 —(NR g )n′-R f , where m, n and n′ are each independently 0 or 1;

Z is O or NR g ;

R f is hydrogen, C 1-12 -alkyl, hydroxy, C 1-12 -alkoxy, amino, C 1-12 -hydroxyalkyl or C 2-12 -alkoxyalkyl, and each R g is independently hydrogen or C 1-12 -alkyl;

R 7 is selected from: hydrogen; C 1-12 -alkyl; C 3-12 -cycloalkyl; C 4-12 -cycloalkylalkyl; C 1-12 -haloalkyl; C 1-12 -haloalkoxy; C 1-12 -hydroxyalky; C 2-12 -alkoxyalkyl; acetyl; C 1-12 -alkylsulfonyl; C 2-12 -alkylsulfonylalkyl; C 2-12 -aminocarbonyloxyalkyl; C 2-12 -hydroxycarbonylalkyl; C 2-12 -hydroxyalkyloxycarbonylalkyl; C 6-12 -aryl; C 7-12 -arylalkyl; C 6-12 -arylsulfonyl; C 5-12 -heteroaryl; C 6-12 -heteroarylalkyl; C 5-12 -heteroarylsulfonyl; C 3-12 -heterocyclyl; and C 4-12 -heterocyclylalkyl;

R d is selected from: hydrogen; C 1-12 -alkyl; C 3-12 -cycloalkyl; C 4-12 -cycloalkylalkyl; C 1-12 -haloalkyl; C 1-12 -haloalkoxy; C 1-12 -hydroxyalky; C 2-12 -alkoxyalkyl; acetyl; C 1-12 -alkylsulfonyl; C 2-12 -alkylsulfonylalkyl; C 2-12 -aminocarbonyloxyalkyl; C 2-12 -hydroxycarbonylalkyl; C 2-12 -hydroxyalkyloxycarbonylalkyl; C 6-12 -aryl; C 7-12 -arylalkyl; C 6-12 -arylsulfonyl; C 5-12 -heteroaryl; C 6-12 -heteroarylalkyl; C 5-12 -heteroarylsulfonyl; C 3-12 -heterocyclyl; and C 4-12 -heterocyclylalkyl;

Q is (CR 9 )x, one of A and E is O, S or NR 10 and the other is (CR 9 )x or N, wherein each x is independently 1 or 2;

or alternatively, Q is N, one of A and E is NR 10 and the other is (CR 9 )x;

each R 9 is independently hydrogen, C 1-12 -alkyl, halo or C 1-12 -alkoxy; and

R 10 is hydrogen, C 1-12 -alkyl, C 1-12 -hydroxyalkyl, C 2-12 -alkoxyalkyl, —(CH 2 )m-(Z)n-(CO)—R f , or —(CH 2 )m-(Z)n-SO 2 —(NR g )n′-R f .

11. The compound of claim 1 of Formula 5, or a pharmaceutically acceptable salt thereof:

wherein:

R 1 is: C 1-12 -alkyl;

R 4 is: hydrogen; C 1-12 -alkyl; C 2-12 -alkenyl; C 2-12 -alkynyl; amino; halo; amido; C 1-12 -haloalkyl; C 1-12 -alkoxy; hydroxy; C 1-12 -haloalkoxy; nitro; C 1-12 -hydroxyalkyl; C 2-12 -alkoxyalkyl; C 1-12 -hydroxyalkoxy; C 3-12 -alkynylalkoxy; C 1-12 -alkylsulfonyl; C 6-12 -arylsulfonyl; cyano; C 6-12 -aryl; C 5-12 -heteroaryl; C 3-12 -heterocyclyl; C 4-12 -heterocyclylalkoxy; C 6-12 -aryloxy; C 5-12 -heteroaryloxy; C 7-12 -arylalkyloxy; C 6-12 -heteroarylalkyloxy; optionally substituted phenoxy; or —(CH 2 )m-(Z)n-(CO)—R f or —(CH 2 )m-(Z)n-SO 2 —(NR g )n′-R f , where m, n and n′ are each independently 0 or 1;

Z is O or NR g ;

R f is hydrogen, C 1-12 -alkyl, hydroxy, C 1-12 -alkoxy, amino, C 1-12 -hydroxyalkyl or C 2-12 -alkoxyalkyl;

each R g is independently hydrogen or alkyl;

R 7 is selected from hydrogen; C 1-12 -alkyl; C 3-12 -cycloalkyl; C 4-12 -cycloalkylalkyl; C 1-12 -haloalkyl; C 1-12 -haloalkoxy; C 1-12 -hydroxyalky; C 2-12 -alkoxyalkyl; acetyl; C 1-12 -alkylsulfonyl; C 2-12 -alkylsulfonylalkyl; C 2-12 -aminocarbonyloxyalkyl; C 2-12 -hydroxycarbonylalkyl; C 2-12 -hydroxyalkyloxycarbonylalkyl; C 6-12 -aryl; C 7-12 -arylalkyl; C 6-12 -arylsulfonyl; C 5-12 -heteroaryl; C 6-12 -heteroarylalkyl; C 5-12 -heteroarylsulfonyl; C 3-12 -heterocyclyl; and C 4-12 -heterocyclylalkyl;

R d is selected from: hydrogen; C 1-12 -alkyl; C 3-12 -cycloalkyl; C 4-12 -cycloalkylalkyl; C 1-12 -haloalkyl; C 1-12 -haloalkoxy; C 1-12 -hydroxyalky; C 2-12 -alkoxyalkyl; acetyl; C 1-12 -alkylsulfonyl; C 2-12 -alkylsulfonylalkyl; C 2-12 -aminocarbonyloxyalkyl; C 2-12 -hydroxycarbonylalkyl; C 2-12 -hydroxyalkyloxycarbonylalkyl; C 6-12 -aryl; C 7-12 -arylalkyl; C 6-12 -arylsulfonyl; C 5-12 -heteroaryl; C 6-12 -heteroarylalkyl; C 5-12 -heteroarylsulfonyl; C 3-12 -heterocyclyl; and C 4-12 -heterocyclylalkyl;

Q is (CR 9 )x, one of A and E is O, S or NR 10 and the other is (CR 9 )x or N, wherein each x is independently 1 or 2;

or alternatively, Q is N, one of A and E is NR 10 and the other is (CR 9 )x;

each R 9 is independently hydrogen, C 1-12 -alkyl, halo or C 1-12 -alkoxy; and

R 10 is hydrogen, C 1-12 -alkyl, C 1-12 -hydroxyalkyl, C 2-12 -alkoxyalkyl, —(CH 2 )m-(Z)n-(CO)—R f , or —(CH 2 )m-(Z)n-SO 2 —(NR g )n′-R f .

12. A method for treating a disease mediated by a P2X3 receptor antagonist, a P2X2/3 receptor antagonist, or both, said method comprising administering to a subject in need thereof an effective amount of a compound of claim 1 , or a pharmaceutically acceptable salt thereof.

13. The compound of claim 2 , or a pharmaceutically acceptable salt thereof, wherein X 5 is CH.

14. The compound of claim 6 , or a pharmaceutically acceptable salt thereof, wherein X 5 is CH.

15. A compound of claim 1 , or a pharmaceutically acceptable salt thereof, selected from:

16. A compound of the following structure, or a pharmaceutically acceptable salt thereof:

Assignments (2)
CHANGE OF ADDRESS Recorded Sep 30, 2022
From: AFFERENT PHARMACEUTICALS, INC.
To: AFFERENT PHARMACEUTICALS, INC.
Reel/Frame 061877/0856 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 14, 2018
From: HAWLEY, RONALD CHARLES; IBRAHIM, PRABHA; FORD, ANTHONY P.; GEVER, JOEL R.
To: AFFERENT PHARMACEUTICALS, INC.
Reel/Frame 046633/0223 →
Continuity (3)
Provisional Application 62363630 · Jul 18, 2016
Provisional Application 62308157 · Mar 14, 2016
Related Publication 20190389811A1 · Dec 26, 2019
Cited By (1)
US 12,605,381