NAD(P)- dependent responsive enzymes, electrodes and sensors, and methods for making and using the same
Embodiments of the present disclosure relate to NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Also provided are methods for making the compositions and for detecting and/or measuring analytes with NADP-dependent oxidoreductase compositions.
1. An enzyme composition comprising:
nicotinamide adenine dinucleotide phosphate (NAD(P) + ) or derivative thereof;
an NAD(P) + -dependent dehydrogenase;
an NAD(P)H oxidoreductase; and
an electron transfer agent comprising a transition metal complex,
wherein the nicotinamide adenine dinucleotide phosphate (NAD(P) + ) or derivative thereof, the NAD(P) + -dependent dehydrogenase, the NAD(P)H oxidoreductase, and the electron transfer agent comprising the transition metal complex are distributed within a single polymer composition.
2. The enzyme composition according to claim 1 , wherein the composition comprises nicotinamide adenine dinucleotide phosphate (NAD(P) + ).
3. The enzyme composition according to claim 1 , wherein the composition comprises a derivative of nicotinamide adenine dinucleotide phosphate.
4. The enzyme composition according to claim 3 , wherein the derivative of nicotinamide adenine dinucleotide phosphate is a compound of Formula I:
wherein X is alkyl, substituted alkyl, aryl, substituted aryl, acyl, and aminoacyl.
5. The enzyme composition according to claim 4 , wherein the derivative of nicotinamide adenine dinucleotide phosphate is:
6. The enzyme composition according to claim 1 , wherein the NAD(P) + -dependent dehydrogenase is selected from the group consisting of glucose dehydrogenase, alcohol dehydrogenase, D-3-hydroxybutyrate dehydrogenase.
7. The enzyme composition according to claim 1 , wherein the NAD(P)H oxidoreductase is diaphorase.
8. The enzyme composition according to claim 1 , wherein electron transfer agent comprises an osmium-containing complex.
9. The enzyme composition according to claim 8 , wherein the osmium-containing complex comprises three heterocyclic nitrogen-containing bidentate ligands.
10. The enzyme composition according to claim 9 , wherein the osmium-containing complex comprises two biimidazole and one imidazole-pyridine bidentate ligands.
11. The enzyme composition according to claim 1 , wherein the polymer composition comprises a heterocycle-containing polymer and a crosslinker.
12. The enzyme composition according to claim 11 , wherein the heterocycle-containing polymer comprises polyvinylpyridine.
13. The enzyme composition according to claim 11 , wherein at least one of the nicotinamide adenine dinucleotide phosphate (NAD(P) + ) or derivative thereof; the NAD(P) + -dependent dehydrogenase; the NAD(P)H oxidoreductase; and the electron transfer agent comprising the transition metal complex are immobilized by the heterocycle-containing polymer.
14. The enzyme composition according to claim 13 , wherein at least one of the nicotinamide adenine dinucleotide phosphate (NAD(P) + ) or derivative thereof; NAD(P) + -dependent dehydrogenase; NAD(P)H oxidoreductase; and redox mediator comprising the transition metal complex are covalently bonded to the heterocycle-containing polymer.
15. The enzyme composition according to claim 14 , wherein the electron transfer agent comprising the transition metal complex is covalently bonded to the heterocycle-containing polymer.
16. The enzyme composition according to claim 1 , comprising:
nicotinamide adenine dinucleotide phosphate (NAD(P) + ) or derivative thereof;
glucose dehydrogenase;
diaphorase; and
an osmium-containing electron transfer agent.
17. The enzyme composition according to claim 1 , comprising:
nicotinamide adenine dinucleotide phosphate (NAD(P) + ) or derivative thereof;
alcohol dehydrogenase;
diaphorase; and
an osmium-containing electron transfer agent.
18. The enzyme composition according to claim 1 , comprising:
nicotinamide adenine dinucleotide phosphate (NAD(P) 30 ) or derivative thereof;
D-3-hydroxybutyrate dehydrogenase;
diaphorase; and
an osmium-containing electron transfer agent.
19. An enzyme composition comprising:
nicotinamide adenine dinucleotide phosphate (NAD(P) + ) or derivative thereof;
an NAD(P) + -dependent dehydrogenase;
an NAD(P)H oxidoreductase; and
an electron transfer agent comprising a transition metal complex,
wherein the nicotinamide adenine dinucleotide phosphate (NAD(P) + ) or derivative thereof, the NAD(P) + -dependent dehydrogenase, the NAD(P)H oxidoreductase, and the electron transfer agent comprising the transition metal complex are uniformly distributed within a single polymer composition.