WHOLE BLOOD STAINING PREPARATION CARTRIDGE AND SYSTEM
A device for facilitating volumetric blood-cell counting includes a blood reservoir configured to receive a blood sample, a first rotatable valve including a first duct having first and second ends, and a first container containing a first reagent. The first end of the first duct is configured to be selectively in fluid communication with the blood reservoir and first container. The device further includes a mixing chamber. The second end of the first duct is configured to be selectively in fluid communication with the mixing chamber.
1 . A device for facilitating volumetric blood-cell counting, comprising:
a blood reservoir configured to receive a blood sample;
a first rotatable valve including a first duct having first and second ends, the first end of the first duct configured to be selectively in fluid communication with the blood reservoir;
a first container containing a first reagent, the first end of the first duct configured to be selectively in fluid communication with the first container; and
a mixing chamber, the second end of the first duct configured to be selectively in fluid communication with the mixing chamber.
2 . The device of claim 1 , further comprising:
a second rotatable valve including a second duct having first and second ends, the first end of the second duct configured to be selectively in fluid communication with the second end of the first duct;
a second container containing a second reagent, the first end of the second duct configured to be selectively in fluid communication with the second container; and
a mixing chamber, the second end of the second duct configured to be selectively in fluid communication with the mixing chamber.
3 . The device of claim 1 , further comprising a housing, wherein the blood reservoir, first rotatable valve, first container and mixing chamber are disposed within the housing.
4 . The device of claim 1 , further comprising a first vacuum port in selective fluid communication with the second end of the first duct.
5 . The device of claim 4 , further comprising a second vacuum port in selective fluid communication with the second end of the first duct.
6 . The device of claim 5 , wherein the first and second vacuum ports comprise hydrophobic membranes.
7 . The device of claim 1 , wherein the first reagent comprises fluorescent dye.
8 . The device of claim 2 , wherein the second reagent comprises red blood cell lyse.