Microfluidic device, system and method
View Patent ↗A combination of components in a capillary flow channel use capillary forces to passively control the movement of liquid samples within a microfluidic device. To detect a target, a liquid sample introduced to a proximal portion of a capillary channel of a microfluidic device moves by capillary action along the specific components of capillary channel.
1. A microfluidic device, comprising:
a capillary flow channel disposed within the microfluidic device, the capillary flow channel comprising a proximal opening and a distal opening;
a filter pocket comprising a filter and disposed at the proximal opening;
a mixing well, disposed distal to the filter pocket;
a dry reagent zone containing a reagent disposed distal to the mixing well;
a pinch region, disposed distal to the dry reagent zone; and
a detection zone, disposed distal to the pinch region,
wherein the distal opening is tapered to control a flow rate and the filter pocket, the mixing well, the dry reagent zone and the pinch region are in fluidic communication, and wherein the pinch region comprises a portion of the capillary flow channel that is lobed in a direction that is substantially perpendicular to a length of the microfluidic device.
2. The microfluidic device of claim 1 , wherein the filter pocket comprises a sample inlet having a recess configured to receive a liquid sample, a filter landing and a vent configured to permit air to be displaced upon receiving the liquid sample.
3. The microfluidic device of claim 2 , wherein the filter landing includes a raised plateau extending from a distal edge of the filter pocket.
4. The microfluidic device of claim 2 , wherein the filter pocket further comprises a catwalk strip disposed for directing a plasma from the sample inlet to the filter landing.
5. The microfluidic device of claim 1 , wherein the mixing well has a bowl shape, having a length, a width, a depth, the mixing well configured to move a filtered liquid sample by capillary action.
6. The microfluidic device of claim 1 , wherein the dry reagent zone contains walls with a hydrophobic ink.
7. The microfluidic device of claim 1 , wherein the pinch region is disposed distal to the dry reagent zone.
8. The microfluidic device of claim 1 , wherein the pinch region is configured with a lobe.
9. The microfluidic device of claim 1 , wherein the detection zone contains at least one solid phase capture spot configured to bind a specific analyte.
10. The microfluidic device of claim 1 , further comprising two or more solid phase capture spots arranged in a series along a length of the detection zone.
11. The microfluidic device of claim 9 , wherein the at least one solid phase capture spot provides a signal measured by a reader.
12. The microfluidic device of claim 10 , wherein at least one solid phase capture spot serves as a control.
13. The microfluidic device of claim 1 , further comprising a waste channel configured to hold excess liquid sample during a wash of the detection zone.
14. The microfluidic device of claim 13 , wherein a portion of a substrate covers the waste channel and that portion is printed with hydrophobic ink to increase flow rate and decrease wash time.
15. The microfluidic device of claim 1 , wherein the capillary flow channel is disposed between an upper substrate and a lower substrate.
16. The microfluidic device of claim 15 , wherein the lower substrate comprises a first portion having a first depth and a second portion having a second depth that is less than the first depth.
17. The microfluidic device of claim 16 , wherein a portion having a first depth is convex and a portion having a second depth is planar.
18. The microfluidic device of claim 1 , wherein the filter pocket is configured to move liquid by capillary action along the capillary flow channel and into the mixing well of the capillary flow channel.
19. The microfluidic device of claim 1 , wherein the mixing well is configured to move liquid by capillary action along the capillary flow channel and into the pinch region of the capillary flow channel.
20. The microfluidic device of claim 1 , wherein the mixing well comprises a depth that is greater than a depth of the capillary flow channel to dampen a concentration of filter components and minimize filter component variation between devices.
21. A method for determining a presence or an absence of a cardiac troponin in a patient sample, comprising:
placing a blood sample on a microfluidic device of claim 1 to label
the cardiac troponin, if present in the patient sample, with a label comprising a binding partner for the cardiac troponin and a detectable moiety; and
detecting a presence of cardiac troponin in the patient sample by determining a presence or an absence of the label, wherein detection of the presence of the label indicates the presence of cardiac troponin in the patient sample, wherein an assay has a limit of quantitation of about 3 pg/mL with a coefficient of variation of less than about 20%.
22. The method of claim 21 , wherein the cardiac troponin is cardiac troponin I (cTnI).
23. The method of claim 21 , wherein the cardiac troponin is cardiac troponin T (cTnT).
24. The method of claim 21 , wherein the cardiac troponin is a complex of cTnI and cTnT.