Immunoassay employing sulfated polysaccharide
Regardless of the type of specimen, such as a serum or heparinized plasma containing different anticoagulants, which are widely used in general, when a substance to be measured (for example, sIL-2R) in a biological sample is immunologically detected, a measuring method and a kit capable of stably obtaining with high accuracy, unaffected by interfering substances in the specimen, are provided. An immunocomplex between the substance to be measured and an antibody that specifically binds to the substance to be measured is formed in the presence of a sulfated polysaccharide. The kit comprises an antibody that specifically binds to the substance to be measured, and a buffer containing a sulfated polysaccharide.
1. A method of immunologically measuring a substance to be measured in a biological sample, said method comprising:
providing a sample selected from the group consisting of a blood sample to which heparin is added as an anticoagulant, and a serum sample;
bringing the sample into contact with an antibody that specifically binds to the substance to be measured in the presence of 0.002% to 0.4% β-cyclodextrin sulfate to form an immunocomplex; and
analyzing the immunocomplex or a signal derived from the immunocomplex.
2. The method according to claim 1 , wherein β-cyclodextrin sulfate is contained in a solution for diluting specimen and/or an antibody solution.
3. The method according to claim 1 , wherein the said analyzing comprises the step of separation of bound and free (B/F) of the formed immunocomplex.
4. The method according to claim 1 , wherein the step of bringing the substance into contact with an antibody comprises contacting with a second antibody that specifically binds to the substance to be measured.
5. The method according to claim 1 , wherein the substance to be measured is soluble interleukin-2 receptor or prostate specific antigen.