Device system and method for fluid sample collection
A system and method for preparing a diagnostic fluid sample for use with a fluid assay system. The method comprises the steps of: amplifying the fluid sample to increase the number of cells subject to being tested; drawing a first volume of the fluid sample into a first syringe of a dual-barrel syringe and dispensing the first volume of the fluid sample through a media filter. The method further comprises the steps of placing the bacteria-laced filter into a lysis module; dispensing a first portion of a lysis buffer into the lysis module from the second syringe of the dual-barrel syringe; and dispensing a second portion of the lysis buffer through the media filter into a disposable cartridge of a fluid assay system.
1. A system for preparing a fluid sample for assay testing of a fluid attribute, comprising:
a dual plunger syringe comprises first and second barrels sharing a common wall, and a rotating valve mechanism containing a nozzle in fluid communication with each of the barrels, the first barrel being empty and configured to receive a quantity of the sample fluid, the second barrel being filled with a lysis buffer;
a filter configured to receive the sample fluid by rotating the valve mechanism to the first barrel and dispensing the sample fluid through the filter, the filter capturing the fluid attribute so as to produce a sample-containing filter,
a lysis module defining a container for receiving the sample-containing filter, the container being filled with the lysis buffer from the second barrel by rotating the valve mechanism of the dual syringe plunger to receive a first portion of the lysis buffer through the nozzle of the valve mechanism, and,
a disposable cartridge operative to receive the fluid sample to assay the fluid attribute, the fluid sample being received within an assay cavity upon opening the lysis module and dispensing a second portion of the lysis buffer from the second barrel through the nozzle of the valve mechanism to flush the sample-containing filter with the second portion of the lysis buffer as the fluid sample is received within the assay cavity.
2. The system of claim 1 , wherein the first and second barrels define first and second volumes, respectively, the first volume being larger than the second volume.
3. The system of claim 1 , wherein the first and second barrels define first and second volumes, respectively, the first volume being five to one-hundred times larger than the second volume.
4. The system of claim 1 , wherein about one-half of the lysis buffer from the second barrel fills the lysis module and about one-half of the lysis buffer from the second barrel flushes the sample containing filter within the lysis module.
5. The system of claim 1 , further comprising a heater operative to heat the lysis module while the lysis buffer breaks down the material of the fluid sample.