TAL-effector mediated herbicide tolerance
The present disclosure relates to methods of altering expression of a genomic locus of interest or specifically targeting a genomic locus of interest in a plant cell, which may involve contacting the genomic locus with a non-naturally occurring or engineered composition that comprises a DNA binding domain comprising one or more Transcription Activator-Like (TAL) effector monomers specifically ordered to target the genomic locus of interest to improve tolerance of the plant cell to an effective concentration of an inhibitor herbicide.
1. A method of conferring to a plant tolerance to an herbicide that is an inhibitor of 4-hydroxyphenylpyruvate dioxygenase (HPPD), said plant comprising an endogenous gene encoding an HPPD enzyme and said method comprising expressing in said plant a nucleic acid molecule comprising a nucleotide sequence encoding a transcription activator-like (TAL) effector, said TAL effector comprising a repeat domain that is engineered for recognition of a region of the endogenous promoter of said HPPD gene, whereby the TAL-effector is capable of increasing the expression of the enzyme from said gene, wherein increased expression of said enzyme in said plant increases its tolerance to said herbicide, wherein said nucleotide sequence is selected from the group consisting of SEQ ID NOs:2-4 and nucleotide sequences encoding the amino acid sequence set forth in any of SEQ ID NOs:6-8.
2. The method of claim 1 , wherein said nucleotide sequence is selected from the group consisting of SEQ ID NO:4 and nucleotide sequences encoding the amino acid sequence set forth in SEQ ID NO:8.
3. A nucleic acid construct comprising a nucleotide sequence encoding an artificial TAL effector and being capable of expression in a plant comprising an endogenous gene encoding an HPPD enzyme, said TAL effector comprising a repeat domain that is engineered for recognition of a region of the endogenous promoter of said HPPD gene, whereby the TAL effector is capable of increasing the expression of said HPPD enzyme from said gene, wherein increased expression of said enzyme in said plant increases its tolerance to an HPPD inhibitor herbicide, wherein said nucleotide sequence is selected from the group consisting of SEQ ID NOs:2-4 and nucleotide sequences encoding the amino acid sequence set forth in any of SEQ ID NOs:6-8.
4. The nucleic acid construct of claim 3 , wherein said nucleotide sequence is selected from the group consisting of SEQ ID NO:4 and nucleotide sequences encoding the amino acid sequence of SEQ ID NO:8.
5. A plant or a plant cell comprising the nucleic acid construct of claim 3 stably incorporated into its genome.
6. The plant of claim 5 , wherein said plant is selected from the group consisting of maize, sorghum, wheat, cabbage, sunflower, tomato, crucifers, peppers, potato, cotton, rice, soybean, sugarbeet, sugarcane, tobacco, barley, and oilseed rape.
7. A seed of the plant of claim 6 , wherein said seed comprises the nucleic acid construct stably incorporated into its genome.