Collagen-coated tissue-based membranes
View Patent ↗A collagen-coated tissue based membrane that is smooth on both sides so as to inhibit cell and tissue adhesion. Also disclosed are methods for making a collagen-coated tissue-based membrane.
1. A method for preparing a biocompatible collagen-coated serous membrane, the method comprising:
scraping a fibrous side of a serous membrane to remove adhering fibers;
treating the serous membrane to remove cells, lipids, and extractable blood and non-collagenous molecules;
freeze-drying the treated serous membrane;
compressing the fibrous side of the serous membrane;
coating the fibrous side of the serous membrane with a collagen; and
exposing the collagen coated serous membrane to a crosslinking agent to effect crosslinking.
2. The method of claim 1 , wherein the serous membrane is rinsed with a dehydrating agent prior to the scraping step.
3. The method of claim 2 , wherein the dehydrating agent is an alcohol.
4. The method of claim 1 , wherein the serous membrane is pericardium, peritoneum, amnion, small intestine submucosa, pleural, or vaginal tunics.
5. The method of claim 1 , wherein the collagen is fiber-forming collagen.
6. The method of claim 5 , wherein the fiber-forming collagen is type I collagen, type II collagen, type III collagen, or a combination thereof.
7. The method of claim 6 , wherein the fiber-forming collagen is type I collagen.
8. The method of claim 1 , wherein the coating is accomplished by spraying the collagen onto the fibrous side of the serous membrane.
9. The method of claim 1 , wherein the coating is accomplished by brushing the collagen onto the fibrous side of the serous membrane.
10. The method of claim 1 , wherein the coating is accomplished by dipping the fibrous side of the serous membrane into the collagen.
11. The method of claim 8 , wherein the serous membrane is pericardium or peritoneum.
12. The method of claim 11 , wherein the collagen is type I collagen.