IP Library Granted Patent US 10,689,610
Granted Patent B2
US 10,689,610 · App. 16/102,448 · Granted Jun 23, 2020

Gas-fed fermentation reactors, systems and processes utilizing gas/liquid separation vessels

Inventors: Luan Thanh Nguyen (San Ramon, CA); Joshua A. Silverman (Los Altos Hills, CA); Graham Ian Aylen (Knowbury, GB)
Assignee: Calysta, Inc.
C12M45/04B01D19/00B01D19/0042C12M21/04C12M21/12C12M23/06C12M29/18C12M29/20
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Quick Facts
Patent No.
US 10,689,610
App. No.
16/102,448
Granted
Jun 23, 2020
Kind
B2
Abstract

Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

Claims (34)

1. A system for stimulating production of biomass comprising:

a loop reactor, the loop reactor including:

a fluid moving device having an inlet and an outlet, the inlet of the fluid moving device in fluid communication with an outlet of a substantially vertical downflow zone;

a loop section including an outlet and an inlet, the inlet of the loop section in fluid communication with the outlet of the fluid moving device;

a substantially vertical upflow zone including an outlet and an inlet, the inlet of the substantially vertical upflow zone in fluid communication with the outlet of the loop section; and

a gas/liquid separation vessel having a longitudinal axis and including:

an outlet and an inlet, the inlet of the gas/liquid separation vessel located in a lower portion of the gas/liquid separation vessel and in fluid communication with the outlet of the substantially vertical upflow zone, the outlet of the gas/liquid separation vessel in fluid communication with an inlet of the substantially vertical downflow zone;

an intermediate section between the outlet and the inlet of the gas/liquid separation vessel, the intermediate section having a constant diameter equal to D;

an outlet side section on one side of the intermediate section, the outlet side section including the outlet of the gas/liquid separation vessel; and

an inlet side section on a side of the intermediate section opposite the outlet side section, the inlet side section including the inlet of the gas/liquid separation vessel, the inlet side section having a shape of an oblique conical frustum and having an increasing diameter in a direction of fluid flow through the inlet side section.

2. The system of claim 1 , further comprising a drain conduit including in inlet end and an outlet end, the inlet end of the drain conduit connected to the outlet side section of the gas/liquid separation vessel and the outlet end of the drain conduit connected to the substantially vertical downflow zone.

3. The system of claim 1 , wherein a lowermost edge of the inlet, the outlet, the intermediate section, the outlet side section and the inlet side section of the gas/liquid separation vessel contact a common plane.

4. The system of claim 1 , further comprising a feed conduit including an inlet and an outlet, the inlet of the feed conduit connected to the substantially vertical upflow zone and the outlet end of the feed conduit connected to the inlet side section of the gas/liquid separation vessel.

5. The system of claim 1 , wherein the inlet side section having a shape of an oblique conical frustum includes a cone angle that ranges between 5 to 30°.

6. The system of claim 1 , wherein the outlet of the gas/liquid separation vessel has a diameter that is less than a diameter of the inlet of the gas/liquid separation vessel.

7. The system of claim 1 , wherein the inlet side section has a length measured along the longitudinal axis of the gas/liquid separation vessel that is greater than a length of the intermediate section measured along the longitudinal axis of the gas/liquid separation vessel and greater than a length of the outlet side section measured along the longitudinal axis of the gas/liquid separation vessel.

8. The system of claim 1 , wherein the outlet side section has a dished shape.

9. The system of claim 1 , wherein the substantially vertical downflow zone has a diameter that is non-increasing.

10. The system of claim 2 , wherein the drain conduit has a diameter that is non-increasing.

11. The system of claim 1 , wherein D ranges from 2 meters to 8 meters.

12. A gas/liquid separation vessel comprising:

an outlet, an inlet, and a longitudinal axis, the inlet of the gas/liquid separation vessel located in a lower portion of the gas/liquid separation vessel;

an intermediate section between the outlet and the inlet of the gas/liquid separation vessel, the intermediate section having a constant diameter equal to D;

an outlet side section on one side of the intermediate section, the outlet side section including the outlet of the gas/liquid separation vessel; and

an inlet side section on a side of the intermediate section opposite the outlet side section, the inlet side section including the inlet of the gas/liquid separation vessel, the inlet side section having a shape of an oblique conical frustum and having an increasing diameter in a direction of fluid flow through the inlet side section.

13. The gas/liquid separation vessel of claim 12 , wherein a lowermost edge of the inlet, the outlet, the intermediate section, the outlet side section and the inlet side section of the gas/liquid separation vessel contact a common plane.

14. The gas/liquid separation vessel of claim 12 , wherein the inlet side section having a shape of an oblique conical frustum includes a cone angle that ranges between 5 to 30°.

15. The gas/liquid separation vessel of claim 12 , wherein the outlet of the gas/liquid separation vessel has a diameter that is less than a diameter of the inlet of the gas/liquid separation vessel.

16. The gas/liquid separation vessel of claim 12 , wherein the inlet side section has a length measured along the longitudinal axis of the gas/liquid separation vessel that is greater than a length of the intermediate section measured along the longitudinal axis of the gas/liquid separation vessel and greater than a length of the outlet side section measured along the longitudinal axis of the gas/liquid separation vessel.

17. The gas/liquid separation vessel of claim 12 , wherein the outlet side section has a dished shape.

18. The gas/liquid separation vessel of claim 12 , wherein D ranges from 2 meters to 8 meters.

19. The gas/liquid separation vessel of claim 12 wherein the intermediate section includes an inlet and an outlet, the inlet of the intermediate section and the outlet of the intermediate section spaced apart by a length L ranging from 2 meters to 8 meters.

20. The gas/liquid separation vessel of claim 12 , wherein the inlet side section having a shape of an oblique conical frustum includes a cone angle that is less than 5°.

21. The gas/liquid separation vessel of claim 12 , wherein the inlet side section having a shape of an oblique conical frustum includes a cone angle that is greater than 30°.

Assignments (4)
RELEASE OF SECURITY INTEREST Recorded Sep 12, 2025
From: VENTURE LENDING & LEASING IX, INC.
To: CALYSTA, INC.
Reel/Frame 072242/0047 →
SECURITY INTEREST Recorded Oct 7, 2024
From: CALYSTA, INC.
To: AQUA-SPARK CALYSTA LLC
Reel/Frame 069116/0150 →
SECURITY INTEREST Recorded Feb 29, 2024
From: CALYSTA, INC.
To: OCEAN 14 CAPITAL FUND 1 SCSP
Reel/Frame 066607/0888 →
SECURITY INTEREST Recorded Aug 27, 2021
From: CALYSTA, INC.
To: VENTURE LENDING & LEASING IX, INC.
Reel/Frame 057337/0355 →