IP Library › Granted Patent US 10,745,365
Granted Patent B2
US 10,745,365 · App. 16/109,146 · Granted Aug 18, 2020

Luminogenic and fluorogenic compounds and methods to detect molecules or conditions

Inventors: James J. Cali (Verona, WI); William Daily (Santa Maria, CA); Erika Hawkins (Madison, WI); Dieter Klaubert (Arroyo Grande, CA); Jianquan Liu (Fremont, CA); Poncho Meisenheimer (San Luis Obispo, CA); Michael Scurria (San Luis Obispo, CA); John W. Shultz (Verona, WI); James Unch (Arroyo Grande, CA); Michael P. Valley (Fitchburg, WI); Keith V. Wood (Mount Horeb, WI); Wenhui Zhou (San Luis Obispo, CA)
Assignee: Promega Corporation
C07D277/66C07D277/68C07D311/16C07D417/04C07D417/14C07D493/10C07F9/65583C09B11/24C09B19/00C09B57/00C09B57/02C12Q1/66G01N2500/00
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Quick Facts
Patent No.
US 10,745,365
App. No.
16/109,146
Granted
Aug 18, 2020
Kind
B2
Abstract

A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.

Claims (7)

1. A method of detecting a luciferase in a sample, comprising:

a) contacting said sample with a reaction mixture comprising a compound having the following structure:

and

b) detecting luminescence in the sample, thereby detecting the luciferase in the sample.

2. The method of claim 1 , wherein the reaction mixture further comprises ATP.

3. The method of claim 1 , wherein the luciferase is firefly luciferase.

4. The method of claim 1 , wherein the luciferase is beetle luciferase.

Assignments (2)
SECURITY INTEREST Recorded Apr 3, 2019
From: PROMEGA CORPORATION; PROMEGA BIOSCIENCES, LLC; TERSO SOLUTIONS, INC.; ORION SEVEN, LLC; PROMEGA AVIATION LLC
To: JPMORGAN CHASE BANK, N.A., AS COLLATERAL AGENT
Reel/Frame 048790/0259 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 2, 2018
From: CALI, JAMES J.; DAILY, WILLIAM; HAWKINS, ERIKA; KLAUBERT, DIETER; LIU, JIANQUAN; MEISENHEIMER, PONCHO; SCURRIA, MICHAEL; SHULTZ, JOHN W.; UNCH, JAMES; VALLEY, MICHAEL P.; WOOD, KEITH V.; ZHOU, WENHUI
To: PROMEGA CORPORATION
Reel/Frame 047040/0757 →