Determination of fertility potential from the oxidation-reduction potential of a biological sample
View Patent ↗Methods and systems for measuring and using the oxidation-reduction potential (ORP) of a biological sample are provided. The system generally includes a test strip and a readout device for determining the ORP. The measured ORP is then used to determine characteristics relating to the fertility of the sample or the subject from which the sample was derived. Some characteristics that can be determined include the quality of a sperm sample, an oocyte or a fertilized egg. The measured ORP value can also be used to determine the specific characteristics of a spermatozoa sample, such as the morphology of the spermatozoa, the motility of the spermatozoa, the number of cells in the sample and the concentration of the cells in the sample. Knowledge of such characteristics and fertility potential can be used to identify individuals that might benefit from specific fertility treatments.
1. A method of evaluating the fertility potential of a successful implantation of a fertilized egg, comprising:
a. measuring the static oxidation-reduction potential (sORP) and/or the capacity oxidation-reduction potential (cORP) of medium in which a fertilized egg is being cultured;
b. comparing the measured sORP and/or cORP of a sample of the medium to a reference value, wherein if the ORP value of the medium being tested is significantly increased compared to the reference value, the fertilized egg cultured in the medium is identified as being unlikely to establish and maintain a successful implantation; and
c. treating the medium with an antioxidant if the ORP value of the medium being tested is significantly increased compared to the reference value.
2. The method of claim 1 , wherein the fertilized egg being cultured is a zygote.
3. The method of claim 1 , wherein the fertilized egg being culture is a blastocyst.
4. The method of claim 1 , wherein the medium is compared every 30 minutes, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 12 hours or 18 hours.
5. The method of claim 1 , wherein the medium is compared every 1 day, 2 days or every 3 days.
6. The method of claim 1 , wherein the reference value is determined from the sORP and/or cORP level in follicular fluid of oocytes.
7. The method of claim 1 , wherein the antioxidant is ascorbic acid.
8. A method of improving the likelihood that a fertilized egg will achieve a successful pregnancy, comprising:
a. measuring the static oxidation-reduction potential (sORP) and/or the capacity oxidation-reduction potential (cORP) of medium in which a fertilized egg is being cultured;
b. comparing the measured sORP and/or cORP of a sample of the medium to a reference value, wherein if the ORP value of the medium being tested is significantly increased compared to the reference value, the fertilized egg cultured in the medium is identified as being unlikely to establish and maintain a successful implantation;
c. treating the medium with an antioxidant if the ORP value of the sample being tested is significantly increased compared to the reference value;
d. comparing a sample of the treated medium of step (c) to the reference value;
e. identifying the fertilized egg cultured in the medium as likely to establish and maintain a successful implantation if the ORP value of the treated medium sample is about the same as the reference value; and
f. implanting the fertilized egg.
9. The method of claim 8 , wherein the fertilized egg being cultured is a zygote.
10. The method of claim 8 , wherein the fertilized egg being culture is a blastocyst.
11. The method of claim 8 , wherein the medium is compared every 30 minutes, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 12 hours or 18 hours.
12. The method of claim 8 , wherein the medium is compared every 1 day, 2 days or every 3 days.
13. The method of claim 8 , wherein the reference value is determined from the sORP and/or cORP level in follicular fluid of oocytes.
14. The method of claim 8 , wherein the antioxidant is ascorbic acid.