Method and kit for generating high affinity binding agents
View Patent ↗A combined ribosome-display and phage-display method and kit for carrying out the method are provided. The method includes screening a ribosome-display library of binding agents to identify binding agents that interact with one or more target molecules of interest, converting the RNA encoding the binding agents to a phage-display format by amplification and primer extension, and the screening the phage-display library to enrich for binding agents that interact with one or more target molecules of interest.
1. A method for generating a high affinity agent to a target molecule comprising:
(a) obtaining a ribosome-display library comprising nucleic acids encoding a population of binding agents;
(b) screening the ribosome-display library for binding agents that bind to one or more target molecules;
(c) amplifying nucleic acids encoding the binding agents that bind to the one or more target molecules;
(d) annealing the amplified nucleic acids of (c) to a phage-display vector comprising nucleic acids encoding at least a portion of the binding agents;
(e) primer extending the annealed nucleic acids of (d) to generate a phage-display library; and
(f) screening the phage-display library to identify phage clones that bind to the one or more target molecules thereby generating high affinity agents.