SOLUTION AND METHOD FOR ADHERING SUSPENSION COMPONENTS TO A SUBSTRATE
This disclosure is directed to a method and an attachment solution for adhering a cytological or histological sample, such as buffy coat, to a substrate, such as a microscope slide. The attachment solution includes an attachment base, an anti-coagulant, and a nonsteroidal anti-inflammatory drug. The attachment base may be an alcohol, an acid, an oxidizer, an organohalogen, a ketone, or any combination thereof. Once a sample is obtained, the sample may be re-suspended in the attachment solution or the attachment solution may be added to the sample. The sample may then be dispensed onto an analysis platform as one or more droplets and cured.
1 . A solution, comprising:
an alcohol; and
a nonsteroidal anti-inflammatory drug having a final concentration of 100-1000 ug/mL,
wherein the final concentration is the concentration of the nonsteroidal anti-inflammatory drug when mixed with a sample.
2 . The solution of claim 1 , wherein the alcohol is methanol.
3 . The solution of claim 2 , wherein the non-steroidal anti-inflammatory drug is acetylsalicylic acid.
4 . The solution of claim 3 , further comprising a buffer.
5 . The solution of claim 3 , further comprising a salt, wherein the solution is hypotonic relative to the sample.
6 . The solution of claim 1 , wherein the alcohol has a final concentration of 60-70% by volume when mixed with the sample.
7 . The solution of claim 1 , further comprising water.
8 . The solution of claim 1 , further comprising a buffered solution.
9 . The solution of claim 1 , further comprising an anti-coagulant.
10 . The solution of claim 9 , wherein the anti-coagulant is heparin.