PRODUCTION OF BIOFUELS, BIOCHEMICALS, AND MICROBIAL BIOMASS FROM CELLULOSIC PULP
Methods for production of alcohol, biomolecules, and/or microbial biomass using cellulosic pulp as a feedstock are provided. Cellulosic pulp is contacted with an enzyme composition to produce fermentable sugars, which are used as a carbon source for a microorganism to produce alcohols or biochemicals by fermentation and/or to produce only microbial biomass.
1 . A method of producing an end product from pulp, the method comprising:
(a) obtaining an aqueous slurry comprising 8 to 12% by weight of cellulosic pulp;
(b) contacting the cellulosic pulp with an enzyme composition to produce fermentable sugars, wherein the aqueous slurry is at a temperature between 45 and 60° C. and a pH between 4.5 and 6; and
(c) using the fermentable sugars as a carbon source for a microorganism to produce an end product.
2 . The method of claim 1 , wherein the cellulosic pulp is brownstock pulp or bleached pulp from a kraft pulping process or a sulfite pulping process.
3 . The method of claim 1 , further comprising adjusting the temperature of the aqueous slurry to between 25 and 38° C. before or during step (c).
4 . The method of claim 1 , wherein the end product is ethanol and wherein step (c) further comprises fermentation of the fermentable sugars by the microorganism and production of ethanol to a concentration of at least 3% w/v in the aqueous slurry.
5 . The method of claim 1 , wherein the end product comprises one or more of the following biochemicals produced by the microorganism: acetic acid, lactic acid, succinic acid, citric acid, gluconic acid, L-ascorbic acid, or itaconic acid.
6 . The method of claim 1 , wherein enzymatic hydrolysis of the cellulosic pulp continues during at least a portion of step (c).
7 . The method of claim 1 , further comprising separating the fermentable sugars from solids in the aqueous slurry before step (c), wherein the end product comprises biomass of the microorganism.
8 . The method of claim 7 , wherein step (c) comprises aerobic propagation of the microorganism and does not comprise anaerobic fermentation of the sugars.
9 . The method of claim 7 , wherein no more than 0.1 g/L of ethanol is produced by the by the microorganism during step (c), and wherein the microorganism is Saccharomyces cerevisiae.
10 . The method of claim 7 , wherein step (c) results in an increase of the biomass of the microorganism by at least 10 fold.
11 . The method of claim 1 , wherein step (c) further comprises providing a nitrogen source to the microorganism.
12 . The method of claim 1 , wherein contacting the cellulosic pulp with the enzyme composition results in the production of glucose to a concentration of at least 5% w/v in the aqueous slurry.
13 . The method of claim 1 , wherein the microorganism is capable of metabolizing five-carbon sugars.
14 . The method of claim 1 , wherein the total weight of the enzyme composition is between 3% and 9% of the dry weight of the cellulosic pulp in the aqueous slurry.
15 . The method of claim 1 , wherein the enzyme composition comprises one or more enzymes selected from the group of enzymes consisting of an endoglucanase, a cellobiohydrolase, a xylanase, and a beta-glucosidase.
16 . The method of claim 7 , wherein step (c) further comprises providing a nitrogen source to the microorganism.
17 . The method of claim 7 , wherein contacting the cellulosic pulp with the enzyme composition results in the production of glucose to a concentration of at least 5% w/v in the aqueous slurry.
18 . The method of claim 7 , wherein the microorganism is capable of metabolizing five-carbon sugars.
19 . The method of claim 7 , wherein the total weight of the enzyme composition is between 3% and 9% of the dry weight of the cellulosic pulp in the aqueous slurry.
20 . The method of claim 7 , wherein the enzyme composition comprises one or more enzymes selected from the group of enzymes consisting of an endoglucanase, a cellobiohydrolase, a xylanase, and a beta-glucosidase.