Compositions comprising cross-species-specific antibodies and uses thereof
The present invention relates to uses of bispecific antibodies exhibiting cross-species specificity for evaluating the in vivo safety and/or activity and/or pharmacokinetic profile of the same in non-human species and humans. The present invention moreover relates to methods for evaluating the in vivo safety and/or activity and/or pharmacokinetic profile of said bispecific antibodies exhibiting cross-species specificity. The present invention also relates to methods of measuring the biological activity and/or efficacy of such bispecific antibodies exhibiting cross-species specificity. In addition, the present invention relates to pharmaceutical compositions comprising bispecific single chain antibodies exhibiting cross-species specificity and to methods for the preparation of pharmaceutical compositions comprising said bispecific single chain antibodies exhibiting cross-species specificity for the treatment of diseases.
1. A bispecific single chain antibody comprising:
(i) a first binding domain which binds to primate CD3 comprising
CDR-L1 (SEQ ID NO: 118), CDR-L2 (SEQ ID NO: 117), and CDR-L3 (SEQ ID NO: 116) and CDR-H1 (SEQ ID NO: 115), CDR-H2 (SEQ ID NO: 114) and CDR-H3 (SEQ ID NO: 112) or (SEQ ID NO: 113),
wherein the first binding domain comprises a VH region comprising the amino acid sequence set forth in SEQ ID NO: 110 and/or a VL region comprising the amino acid sequence set forth in SEQ ID NO: 168; and
(ii) a second binding domain binding to a cell surface antigen.
2. The bispecific single chain antibody of claim 1 , wherein said first binding domain binds to an epitope of human and non-chimpanzee primate CD3 comprising the amino acid sequence of “FSEXE” (SEQ ID NO: 204), wherein “X” represents L (Leucine) or M (Methionine).
3. The bispecific single chain antibody of claim 1 , wherein said first binding domain is located C-terminally or N-terminally to the second binding domain.
4. The bispecific single chain antibody of claim 1 , wherein the second binding domain binds to a human cell surface antigen and to a non-chimpanzee primate homolog of the cell surface antigen.
5. The bispecific single chain antibody of claim 1 , wherein the cell surface antigen is a tumor antigen.
6. The bispecific single chain antibody of claim 1 , wherein the first binding domain comprises a VH region comprising the amino acid sequence set forth in SEQ ID NO: 110.
7. The bispecific single chain antibody of claim 1 , wherein the first binding domain comprises a VL region comprising the amino acid sequence set forth in SEQ ID NO: 168.
8. The bispecific single chain antibody of claim 1 , wherein the VH region of the first binding domain comprises or consists of the amino acid sequence set forth in SEQ ID NO: 110 and the VL region of the first binding domain comprises or consists of the amino acid sequence set forth in SEQ ID NO: 168.
9. The bispecific single chain antibody of claim 5 , wherein said tumor antigen is EpCAM, EGFR, EGFRvIII or Carboanhydrase IX (MN/CA IX).
10. The bispecific single chain antibody of claim 1 , wherein the primate is a baboon, marmoset or an old world monkey.
11. The bispecific single chain antibody of claim 10 , wherein the old world monkey is a monkey of the macaque genus.
12. The bispecific single chain antibody of claim 1 , wherein the primate CD3 comprises or consists of the amino acid sequence set forth in SEQ ID NO: 135, 136, 144 or 145.
13. The bispecific single chain antibody of claim 1 , wherein at least one of said first or second binding domains is human, humanized, CDR-grafted and/or deimmunized.
14. A composition comprising the bispecific single chain antibody of claim 1 and a carrier, stabilizer, and/or excipient.
15. The bispecific single chain antibody of claim 1 , wherein the first binding domain comprises a single chain antibody fragment (scFv) comprising:
(a) the amino acid sequence set forth in SEQ ID NO: 170; or
(b) an amino acid sequence encoded by the nucleotide sequence set forth in SEQ ID NO: 169.
16. A bispecific single chain antibody comprising:
(i) a first binding domain which binds to primate CD3 comprising CDR-L1 (SEQ ID NO: 118), CDR-L2 (SEQ ID NO: 117), and CDR-L3 (SEQ ID NO: 116) and CDR-H1 (SEQ ID NO: 115), CDR-H2 (SEQ ID NO: 114) and CDR-H3 (SEQ ID NO: 112) or (SEQ ID NO: 113),
wherein the first binding domain comprises a single chain antibody fragment (scFv) comprising:
(a) the amino acid sequence set forth in SEQ ID NO: 194; or
(b) an amino acid sequence encoded by the nucleotide sequence set forth in SEQ ID NO: 193; and
(ii) a second binding domain binding to a cell surface antigen.