TAILORED OILS PRODUCED FROM RECOMBINANT OLEAGINOUS MICROORGANISMS
Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
1 .- 114 . (canceled)
115 . A method of producing a triglyceride oil, the method comprising:
a. cultivating a recombinant oleaginous microbe, the microbe comprising an exogenous gene that encode a β-ketoacyl synthase II (KASII) peptide;
b. recovering the triglyceride oil from said recombinant oleaginous microbe; and
wherein the triglyceride oil is enriched in C18 fatty acids or reduced in C16 fatty acids.
116 . The method of claim 115 , wherein the triglyceride oil is enriched in C18:0 and C18:1 fatty acids.
117 . The method of claim 115 , wherein the triglyceride is reduced in C16:0 fatty acids.
118 . The method of claim 115 , wherein the triglyceride oil comprises at least 60% C18:1.
119 . The method of claim 115 , wherein the recombinant oleaginous microbe is microalgae.
120 . The method of claim 119 , wherein the microalgae is the genus Prototheca or Chlorella.
121 . The method of claim 120 , wherein the microalgae is Prototheca moriformis.
122 . The method of claim 119 , wherein the exogenous nucleic acids are codon-optimized for expression in microalgae.
123 . The method of claim 122 , wherein the codon optimized nucleic acids contains the most or second most preferred codon of Table 2 for at least 60% of the codons of the coding sequence.
124 . The method of claim 115 , wherein the recombinant oleaginous microbe comprises a second exogenous gene, wherein the second exogenous encodes a sucrose invertase.
125 . A triglyceride oil produced by the method of claim 115 .
126 . A recombinant oleaginous microbial cell, the recombinant microbial cell comprising an exogenous gene that encodes a β-ketoacyl synthase II (KASII) peptide, wherein fatty acids of triglyceride oil produced by the recombinant microbial cell is enriched in C18 fatty acids or reduced in C16 fatty acids.
127 . The recombinant oleaginous microbial cell of claim 126 , wherein the triglyceride oil is enriched in C18:0 and C18:1 fatty acids.
128 . The recombinant oleaginous microbial cell of claim 127 , wherein the triglyceride oil comprises at least 60% C18:1.
129 . The recombinant oleaginous microbial cell of claim 126 , wherein the recombinant oleaginous microbe is microalgae.
130 . The recombinant oleaginous microbial cell of claim 129 , wherein the microalgae is the genus Prototheca or Chlorella.
131 . The recombinant oleaginous microbial cell of claim 130 , wherein the microalgae is Prototheca moriformis.