Corn event 5307
A novel transgenic corn event designated 5307, is disclosed. The invention relates to DNA sequences of the recombinant constructs inserted into the corn genome and of genomic sequences flanking the insertion site that resulted in the 5307 event. The invention further relates to assays for detecting the presence of the DNA sequences of event 5307, to corn plants and corn seeds comprising the genotype of and to methods for producing a corn plant by crossing a corn plant comprising the event 5307 genotype with itself or another corn variety.
1. A method of producing corn seed, the method comprising:
(a) sexually crossing a first inbred corn plant with a second inbred corn plant, wherein the first corn plant or the second corn plant comprises corn event 5307 DNA, and wherein representative seed comprising the corn event 5307 DNA has been deposited at the American Type Culture Collection under the accession number PTA-9561; and
(b) harvesting the resultant first generation corn seed.
2. A method of producing hybrid corn seeds, the method comprising:
a) planting seeds of a first inbred corn plant comprising event 5307 DNA and seeds of a second inbred corn plant having a genotype different from the first inbred corn plant, wherein representative seed comprising the corn event 5307 DNA has been deposited at the American Type Culture Collection under the accession number PTA-9561;
b) cultivating corn plants resulting from said planting until the production of flowers;
c) emasculating said flowers produced at the time of flowering of either the first or the second inbred corn line;
d) sexually crossing the non-emasculated inbred plant with pollen of the emasculated inbred plant to produce hybrid corn seeds; and
e) harvesting the hybrid corn seeds.
3. Hybrid corn seed produced by the method of claim 2 .
4. Hybrid corn plants produced by growing hybrid corn seed of claim 3 .
5. A method for producing a corn plant resistant to a coleopteran insect pest comprising:
(a) sexually crossing a first parent corn plant with a second parent corn plant, wherein said first or second parent corn plant comprises corn event 5307 DNA, thereby producing a plurality of first generation progeny plants, and wherein representative seed comprising the corn event 5307 DNA has been deposited at the American Type Culture Collection under the accession number PTA-9561;
(b) selecting a first generation progeny plant that is resistant to a coleopteran insect pest infestation;
(c) selfing the first generation progeny plant, thereby producing a plurality of second generation progeny plants; and
(d) selecting from the second generation progeny plants, a plant that is resistant to a coleopteran insect pest;
wherein the selected second generation progeny plant comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4.
6. The method according to claim 5 , wherein the coleopteran insect pest comprises a corn rootworm.
7. The method according to claim 5 , wherein the coleopteran insect pest comprises western corn rootworm.