Fluorescent dyes and methods of use thereof
Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.
1. A composition of matter, comprising:
a first PCR primer of a PCR primer pair, said first PCR primer comprising a first oligonucleotide covalently labeled with Dye 1 or Dye 7.
2. The composition of matter of claim 1 , wherein the first oligonucleotide is covalently labeled with Dye 1.
3. The composition of matter of claim 2 , further comprising:
a second PCR primer of the PCR primer pair, said second PCR primer comprising a second oligonucleotide.
4. The composition of matter of claim 3 , wherein said second oligonucleotide is covalently labeled with a fluorophore.
5. The composition of matter of claim 4 , wherein said fluorophore is fluorescein.
6. The composition of matter of claim 1 , wherein the first oligonucleotide is covalently labeled with Dye 7.
7. The composition of matter of claim 6 , further comprising:
a second PCR primer of the PCR primer pair, said second PCR primer comprising a second oligonucleotide.
8. The composition of matter of claim 7 , wherein said second oligonucleotide is covalently labeled with a fluorophore.
9. The composition of matter of claim 8 , wherein said fluorophore is fluorescein.
10. A polynucleic acid molecule comprising nucleotides wherein at least one of said nucleotides is labeled with Dye 1 or Dye 7.
11. The polynucleic acid molecule of claim 10 , wherein at least one of said nucleotides is labeled with Dye 1.
12. The polynucleic acid molecule of claim 10 , wherein at least one of said nucleotides is labeled with Dye 7.