Cell and tissue culture container
The invention generally relates to containers for cell and tissue culturing with multiple compartments in fluid communication with each other to provide a common culture environment in each of the compartments while maintaining physical separation of cells and tissue therein. The invention further relates to culture containers providing a sterile culture environment with detachably coupleable lids and open access to each compartment within a container.
1. A method for culturing a tissue comprising:
providing a container comprising a bottom wall coupled to a bottom edge of at least one side wall, and an at least one interior wall coupled to the at least one side wall to define at least a first compartment and a second compartment within the container, wherein the at least one interior wall comprises a bottom edge that does not contact the bottom wall, leaving a gap between the bottom wall and the at least one interior wall, wherein the gap allows fluid to pass between the first and second compartments without allowing cells, tissues, or 3D scaffolds to pass therebetween;
providing a lid comprising a gas-permeable membrane, wherein the lid is configured to be detachably coupled to a top edge of the at least one side wall;
introducing a first living cell of a first cell type to the first compartment and a second living cell of a second cell type to the second compartment in the presence of a cell culture medium;
exposing the container to conditions promoting cell growth to produce a first tissue from the first living cell and a second tissue from the second living cell.
2. The method of claim 1 , wherein the first cell type or the second cell type is a chondrocyte, mesenchymal stem cell, fibroblast, osteocyte, osteoblast, synoviocyte, induced pluripotent stem cell (iPSC), embryonic stem cell (ESC), lymphocyte, adipocyte, myofibroblast, hepatocyte, islet cell, monocyte, endometrial regenerative cell, or cancer stem cell.
3. The method of claim 2 , wherein the first cell type is a chondrocyte.
4. The method of claim 3 , wherein the first tissue is neo-cartilage.
5. The method of claim 2 , wherein the second cell type is a chondrocyte.
6. The method of claim 5 , wherein the second cell type is neo-cartilage.
7. The method of claim 1 , wherein the first living cell or the second living cell is present on the bottom wall of the container.
8. The method of claim 1 , wherein the exposing step comprises adding a fluid to a level below the height of the at least one interior wall.
9. The method of claim 1 , further comprising seeding a 3D scaffold with the first living cell and introducing the 3D scaffold seeded with the first living cell into the first compartment.
10. The method of claim 1 , further comprising preparing the first tissue for implantation into a human body.
11. The method of claim 1 , further comprising performing testing on the second tissue to verify viability of the first tissue.
12. The method of claim 1 , wherein the lid or the container comprises polypropylene, high-density polyethylene, polystyrene, styrene-acrylonitrile (SAN), polycarbonatestyrene-maleic anhydride (SMA), cyclic olefin copolymers (COC), acrylic, acrylic-polyvinyl chloride alloys, polypropylene copolymers, polysulfone, polymethylpentene, or cellulosic.
13. The method of claim 1 , wherein the container comprises a circular bottom wall, a circular lid, and a single, curved sidewall coupled thereto to form a substantially cylindrical shape.
14. The method of claim 1 , wherein the container comprises at least 3 compartments.
15. The method of claim 1 , wherein the at least one side wall has a first height relative to the bottom wall that is substantially equal to a second height of the at least one interior wall relative to the bottom wall.
16. The method of claim 1 , wherein the gas-permeable membrane comprises a 0.2 micron filter.
17. The method of claim 1 , wherein the container is configured to hold at least 500 mL of fluid.