Optical lens system and method for microfluidic devices
In some embodiments, a variety of devices and methods for conducting microfluidic analyses are utilized herein, including devices that can be utilized to conduct thermal cycling reactions such as nucleic acid amplification reactions. The devices include elastomeric components; in some instances, much or all of the device is composed of elastomeric material. Amplification products (amplicons) can be detected and distinguished (whether isolated in a reaction chamber or at any subsequent time) using routine methods for detecting nucleic acids. An example of a detection method includes hybridization to arrays of immobilized oligo or polynucleotides.
1. A method of detecting a plurality of reactions in a microfluidic device, the method comprising:
i) flowing a plurality of samples into reaction chambers of the microfluidic device;
ii) amplifying template nucleic acids from the plurality of samples;
iii) detecting amplification reactions;
wherein the method is performed by a system for detecting a plurality of reactions in a microfluidic device, the system comprising:
an automated pressure source for applying a pressure to actuate valves in an elastomeric microfluidic device and to introduce samples into a plurality of reaction chambers of the elastomeric microfluidic device, wherein the elastomeric microfluidic device comprises a carrier accessible to the automated pressure source;
a thermal platen configured to mate with a portion of the carrier of the elastomeric microfluidic device; and
an optical imaging system comprising a light source, an optical lens system, and a detector array camera, wherein:
the automated pressure source, the thermal platen, and the optical imagining system are part of a single platform.
2. The method of claim 1 , further comprising isolating at least some of the plurality of the reaction chambers from one another.
3. The method of claim 1 , wherein the microfluidic device is configured to react M different samples with N different reagents.
4. A system for detecting a plurality of reactions in a microfluidic device, comprising:
an automated pressure source for applying a pressure to actuate valves in an elastomeric microfluidic device and to introduce samples into a plurality of reaction chambers of the elastomeric microfluidic device, wherein the elastomeric microfluidic device comprises a carrier accessible to the automated pressure source;
a thermal platen configured to mate with a portion of the carrier of the elastomeric microfluidic device; and
an optical imaging system comprising a light source, an optical lens system, and a detector array camera, wherein;
the automated pressure source, the thermal platen, and the optical imaging system are part of a single platform.
5. The system of claim 4 , wherein a plurality of reaction chambers of the microfluidic device each comprise an array of immobilized oligo or polynucleotides.
6. The system of claim 5 , wherein at least some of the plurality of the reaction chambers are isolatable from one another.
7. The system of claim 5 , wherein primers are deposited into the plurality of reaction chambers.
8. The system of claim 5 , wherein amplification reagents are flowable into the plurality of reaction chambers.
9. The system of claim 4 , wherein the microfluidic device is configured to react M different samples with N different reagents.
10. The system of claim 4 , wherein the system is configured to perform real time amplification methods within the microfluidic device.
11. The system of claim 4 , wherein the thermal platen is disposed on the opposite side of the elastomeric microfluidic device as the optical imaging system.
12. The system of claim 4 , wherein the detector array camera is a CCD array camera.