Methods and kits for detection of coenzyme Q10
The invention provides methods for rapid and quantitative extraction and detection of coenzyme Q10 in a sample readily adaptable to high throughput screening methods. The invention further provides reagents and kits for practicing the methods of the invention.
1. An isotopically labeled analog of the oxidized form of coenzyme Q10 CoQ10-d6 of the following formula:
2. An isotopically labeled analog of the reduced form of coenzyme Q10 CoQ10H 2 -d6 of the following formula:
3. A mixture for analysis by LC/MS/MS comprising a biological sample and the isotopically labeled analog of claim 1 .
4. The mixture of claim 3 , wherein said biological sample is a bodily fluid.
5. The mixture of claim 4 , wherein said bodily fluid is blood.
6. The mixture of claim 3 , wherein said biological sample is serum.
7. The mixture of claim 3 , wherein said biological sample is plasma.
8. The mixture of claim 3 , wherein said biological sample is a human sample.
9. A mixture for analysis by LC/MS/MS comprising a biological sample and the isotopically labeled analog of claim 2 .
10. The mixture of claim 9 , wherein said biological sample is a bodily fluid.
11. The mixture of claim 10 , wherein said bodily fluid is blood.
12. The mixture of claim 9 , wherein said biological sample is serum.
13. The mixture of claim 9 , wherein said biological sample is plasma.
14. The mixture of claim 9 , wherein said biological sample is a human sample.