CELL COMPOSITION AND METHODS OF MAKING THE SAME
Provided herein are improved methods for the formulation of compositions comprising placental stem cells, and improved compositions and cell formulations produced thereby.
1 - 28 . (canceled)
29 . A composition comprising a plurality of isolated human adherent placental cells in a solution comprising 10% dextran 40, wherein said composition comprises between about 1.0±0.3×106 cells per milliliter to about 5.0±1.5×106 cells per milliliter, and wherein said composition comprises no macro cell clumps.
30 . The composition of claim 29 , wherein said placental stem cells are thawed cryopreserved cells.
31 . The composition of claim 29 , wherein said composition is contained within a bag.
32 . The composition of claim 29 , wherein said composition comprises no macro cell clumps.
33 . The composition of claim 29 , wherein said composition comprises fewer than about 200 micro cell clumps per 106 cells.
34 . The composition of claim 29 , wherein said composition comprises fewer than about 150 micro cell clumps per 106 cells.
35 . The composition of claim 29 , wherein said composition comprises fewer than about 100 micro cell clumps per 106 cells.
36 . The composition of claim 29 , wherein said isolated human adherent placental cells are CD10+, CD34− and CD105+.
37 . The composition of claim 36 , wherein said CD10+, CD34− and CD105+ cells are CD200+.
38 . The composition of claim 37 , wherein said CD10+, CD34−, and CD105+ and CD200+ cells are either CD45− or CD90+.
39 . The composition of claim 37 , wherein said CD10+, CD34−, and CD105+ and CD200+ cells are CD45− or CD90+.
40 . The composition of claim 29 , wherein said composition is a pharmaceutical composition.
41 - 42 . (canceled)