Plant promoter for transgene expression
This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Glycine max egg cell gene. Some embodiments relate to a promoter or a 5′ UTR from a Glycine max egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR or a terminator from a Glycine max egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.
1. A nucleic acid vector comprising a promoter operably linked to:
a) a non-natural synthetic polylinker sequence;
b) a heterologous coding sequence; or
c) a combination of a) and b); wherein said promoter comprises a polynucleotide sequence that has at least 99% sequence identity with SEQ ID NO:2, and wherein said promoter has ovule preferred tissue expression.
2. The nucleic acid vector of claim 1 , wherein said promoter is 1,089 bp in length.
3. The nucleic acid vector of claim 1 , wherein said promoter consists of a polynucleotide sequence that has at least 99% sequence identity with SEQ ID NO:2.
4. The nucleic acid vector of claim 1 , wherein said promoter is operably linked to a heterologous coding sequence.
5. The nucleic acid vector of claim 4 , wherein the heterologous coding sequence encodes a selectable marker protein, an insecticidal resistance protein, a herbicide tolerance protein, a nitrogen use efficiency protein, a water use efficiency protein, a small RNA molecule, a nutritional quality protein, or a DNA binding protein.
6. The nucleic acid vector of claim 4 , further comprising a terminator polynucleotide sequence operably linked to the heterologous coding sequence.
7. The nucleic acid vector of claim 4 , further comprising a 3′ untranslated polynucleotide sequence operably linked to the heterologous coding sequence.
8. The nucleic acid vector of claim 1 , further comprising a 5′ untranslated polynucleotide sequence operably linked to the promoter.
9. The nucleic acid vector of claim 1 , further comprising an intron sequence operably linked to the promoter.
10. A transgenic plant comprising a polynucleotide sequence that has at least 99% sequence identity with SEQ ID NO:2 operably linked to a heterologous coding sequence, wherein said promoter has ovule preferred tissue expression.
11. The transgenic plant of claim 10 , wherein said plant is selected from the group consisting of Zea mays , wheat, rice, sorghum, oats, rye, bananas, sugar cane, Glycine max , cotton, Arabidopsis , tobacco, sunflower, and canola.
12. The transgenic plant of claim 11 , wherein said plant is Glycine max.
13. The transgenic plant of claim 10 , wherein the heterologous coding sequence is inserted into the genome of said plant.
14. The transgenic plant of claim 10 , further comprising a 3′ untranslated sequence operably linked to the heterologous coding sequence.
15. The transgenic plant of claim 10 , wherein said polynucleotide sequence is 1,089 bp in length.
16. A method for producing a transgenic plant cell, the method comprising the steps of:
a) transforming a plant cell with a gene expression cassette comprising a Glycine max egg cell promoter, wherein the Glycine max egg cell promoter comprises a polynucleotide sequence that has at least 99% sequence identity with SEQ ID NO:2, operably linked to at least one polynucleotide sequence of interest;
b) isolating the transformed plant cell comprising the gene expression cassette; and,
c) producing a transgenic plant cell comprising the Glycine max egg cell promoter of claim 1 operably linked to at least one polynucleotide sequence of interest, and wherein said promoter has ovule preferred tissue expression.
17. A method for expressing a polynucleotide sequence of interest in a plant cell, the method comprising introducing into the plant cell a polynucleotide sequence of interest operably linked to a Glycine max egg cell promoter, wherein the Glycine max egg cell promoter comprises a polynucleotide sequence that has at least 99% sequence identity with SEQ ID NO:2, and wherein said promoter has ovule preferred tissue expression.