Agricultural endophyte-plant compositions, and methods of use
This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant or a Glycine plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that include novel seed bacterial endophytes, and synthetic combinations of seeds and/or seedlings (e.g., cereal or soy seeds and/or cereal or soy seedlings) with heterologous seed-derived bacterial endophytes.
1. A synthetic combination comprising a purified bacterial population in association with a Glycine agricultural plant seed, wherein the purified bacterial population is heterologously disposed on the seed surface and comprises a Burkholderia bacterial endophyte comprising a 16S rDNA sequence comprising SEQ ID NO:1446 and capable of producing or inducing production of an auxin or an acetoin in a Glycine agricultural plant produced from the synthetic combination, and wherein the bacterial endophyte is present in the synthetic combination in an amount effective to provide a benefit to the seed or the Glycine agricultural plant produced from the synthetic combination compared to a reference plant produced from a reference seed.
2. The synthetic combination of claim 1 , wherein the purified bacterial population comprises a plurality of bacterial endophyte entities.
3. The synthetic combination of claim 1 , wherein the benefit is increased tolerance to drought.
4. An article of manufacture, comprising: packaging material and a plurality of the synthetic combination of claim 1 .
5. The synthetic combination of claim 1 , further comprising at least one of an adhesion agent, a pesticide, and a fertilizer.
6. The synthetic combination of claim 1 , further comprising a purified fungal population.
7. The synthetic combination of claim 1 , wherein the bacterial population is obtained from an interior seed compartment.
8. The synthetic combination of claim 1 , wherein the bacterial population is obtained from a plant species other than Glycine.
9. The synthetic combination of claim 1 , wherein the bacterial population is obtained from a surface sterilized seed.
10. The synthetic combination of claim 1 , wherein the synthetic combination and/or the Glycine agricultural plant derived therefrom comprises an increased production of a polypeptide of Aspartate aminotransferase glyoxysomal isozyme AAT1 precursor compared to the reference seed and/or the reference plant grown from the reference seed.
11. The synthetic combination of claim 1 , wherein the bacterial endophyte is amplified within a tissue of the Glycine agricultural plant derived from the synthetic combination.
12. The synthetic combination of claim 1 , wherein the bacterial endophyte colonizes roots of the Glycine agricultural plant derived from the synthetic combination.
13. The synthetic combination of claim 1 , wherein the effective amount is at least 10{circumflex over ( )}4 CFU of the bacterial endophyte on the seed surface.
14. The synthetic combination of claim 1 , wherein the Glycine seed is a Glycine max seed.
15. The synthetic combination of claim 3 , wherein the increased tolerance to drought occurs under conditions of biotic stress.
16. The synthetic combination of claim 3 , wherein the increased tolerance to drought occurs under conditions of biotic stress and the biotic stress is selected from the group consisting of nematode stress, insect herbivory stress, fungal pathogen stress, bacterial pathogen stress, and viral pathogen stress.
17. The article of manufacture of claim 4 , wherein the plurality of the synthetic combination are packaged in the packaging material such that they are substantially stable at about 25 degrees C. for at least 7 days.
18. The article of manufacture of claim 4 , wherein the packaging material is selected from a bag, box, bin, envelope, carton, or container.
19. The article of manufacture of claim 4 , wherein the packaging material comprises a desiccant.
20. The article of manufacture of claim 4 , wherein the packaging material comprises an anti-fungal agent.